Quantitation of molecular densities by cryo-electron microscopy : Determination of the radial density distribution of tobacco mosaic virus
dc.contributor.author | Smith, Michael F. | en_US |
dc.contributor.author | Langmore, John P. | en_US |
dc.date.accessioned | 2006-04-10T15:07:28Z | |
dc.date.available | 2006-04-10T15:07:28Z | |
dc.date.issued | 1992-08-05 | en_US |
dc.identifier.citation | Smith, Michael F., Langmore, John P. (1992/08/05)."Quantitation of molecular densities by cryo-electron microscopy : Determination of the radial density distribution of tobacco mosaic virus." Journal of Molecular Biology 226(3): 763-774. <http://hdl.handle.net/2027.42/29906> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WK7-4DPBWPM-6C/2/012f5aa8947d565ed498e6c9cbf17ba9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29906 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1507225&dopt=citation | en_US |
dc.description.abstract | We have determined the absolute mass and radial scattering density distribution of tobacco mosaic virus in the frozen-hydrated state by energy-filtered low-dose bright-field transmission electron microscopy. The absolute magnitude of electron scattering from tobacco mosaic virus in 150 nm of ice was within 3.0% of that predicted, with inelastic scattering accounting for ~80% of the scattering contrast. In order to test the accuracy of the radial reconstruction, a computer model of tobacco mosaic virus was built from the atomic co-ordinates assuming uniform solvent density. The validity of the model was confirmed by comparison of X-ray scattering and predictions of the model (R FACTOR = 0.05). First-order corrections for the microscope contrast transfer function were necessary and sufficient for conversion of the cryo-electron microscopy images into accurate representations of the mass density. At 1.9 nm resolution the compensated reconstruction and model had density peaks of similar magnitude at 2.4, 4.2, 6.0 and 7.8 nm radius and a central hole of 2 nm radius. Equatorial Fourier transforms of the corrected electron images were in excellent agreement with predictions of the model (R FACTOR = 0.12). Thus, the uniform solvent approximation was adequate at 1.9 nm resolution to describe quantitatively X-ray scattering in liquid water and electron imaging in vitreous ice. This is the first demonstration that cryo-electron microscopy images can be used to quantitate the absolute mass, mass per unit length and internal density distributions of proteins and nucleic acids. | en_US |
dc.format.extent | 2686127 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Quantitation of molecular densities by cryo-electron microscopy : Determination of the radial density distribution of tobacco mosaic virus | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Biophysics Research Division and Department of Biological Sciences, The University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109-2099, U.S.A. | en_US |
dc.contributor.affiliationum | Biophysics Research Division and Department of Biological Sciences, The University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109-2099, U.S.A. | en_US |
dc.identifier.pmid | 1507225 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29906/1/0000263.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-2836(92)90631-S | en_US |
dc.identifier.source | Journal of Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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