Isolation and partial characterization of lipoprotein A-II (LP-A-II) particles of human plasma
dc.contributor.author | Bekaert, Etienne D. | en_US |
dc.contributor.author | Alaupovic, Petar | en_US |
dc.contributor.author | Knight-Gibson, Carolyn | en_US |
dc.contributor.author | Norum, Robert A. | en_US |
dc.contributor.author | Laux, Matthew J. | en_US |
dc.contributor.author | Ayrault-Jarrier, M. | en_US |
dc.date.accessioned | 2006-04-10T15:10:44Z | |
dc.date.available | 2006-04-10T15:10:44Z | |
dc.date.issued | 1992-06-05 | en_US |
dc.identifier.citation | Bekaert, Etienne D., Alaupovic, Petar, Knight-Gibson, Carolyn, Norum, Robert A., Laux, Matthew J., Ayrault-Jarrier, M. (1992/06/05)."Isolation and partial characterization of lipoprotein A-II (LP-A-II) particles of human plasma." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1126(1): 105-113. <http://hdl.handle.net/2027.42/29986> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-48897DF-5K/2/18dae8bca6f2819c8451e0ef71306e12 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29986 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1606170&dopt=citation | en_US |
dc.description.abstract | High density lipoproteins (HDL) consist of a mixture of chemically and functionally distinct families of particles defined by their characteristic apolipoprotein (Apo) composition. The two major lipoprotein families are lipoprotein A-I (LP-A-I) and lipoprotein A-I : A-II (LP-A-I : A-II). This study describes the isolation of a third minor HDL family of particles referred to as lipoprotein A-II (LP-A-II) because it lacks ApoA-I and contains ApoA-II as its main or sole apolipoprotein constituent. Because ApoA-II is an integral protein constituent of three distinct lipoprotein families (LP-A-I : A-II, LP-A-II : B : C : D : E and LP-A-II), LP-A-II particles were isolated from whole plasma by sequential immunoaffinity chromatography on immunosorbers with antisera to ApoA-II, ApoB and ApoA-I, respectively. In normolipidemic subjects, the concentration of LP-A-II particles, based on ApoA-II content, is 4-18 mg/dl accounting for 5-20% of the total ApoA-II not associated with ApoB-containing lipoproteins. The lipid composition of LP-A-II particles is characterized by low percentages of triglycerides and cholesterol esters and a high percentage of phospholipids in comparison with lipid composition of LP-A-I and LP-A-I : A-II. The major part of LP-A-II particles contain ApoA-II as the sole apolipoprotein constituents; however, small subsets of LP-A-II particles may also contain ApoD and other minor apolipoproteins. The lipid/protein ratio of LP-A-II is higher than those of LP-A-I and LP-A-I : A-II. In homozygous ApoA-I and ApoA-I/ApoC-III deficiencies, LP-A-II particles are the only ApoA-containing high density lipoprotein with levels found to be within the same range (7-13 mg/dl) as those of normolipidemic subjects. However, in contrast to normal LP-A-II, their lipid composition is characterized by higher percentage of triglycerides and cholesterol esters and a lower percentage of phospholipids and their apolipoprotein composition by the presence of ApoC-peptides and ApoE in addition to ApoA-II and ApoD. These results show that LP-A-II particles are a minor HDL family and suggest that, in the absence of ApoA-I-containing lipoproteins, they become an efficient acceptor/donor of ApoC-peptides and ApoE required for a normal metabolism of triglyceride-rich lipoproteins. Their other possible functional roles in lipid transport remain to be established in future experiments. | en_US |
dc.format.extent | 857778 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation and partial characterization of lipoprotein A-II (LP-A-II) particles of human plasma | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Health Center, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Lipoprotein and Atherosclerosis Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA | en_US |
dc.contributor.affiliationother | Lipoprotein and Atherosclerosis Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA | en_US |
dc.contributor.affiliationother | Lipoprotein and Atherosclerosis Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA | en_US |
dc.contributor.affiliationother | Department of Dermatology, Henry Ford Hospital, Detroit, Mi, USA | en_US |
dc.contributor.affiliationother | INSERM U32, Hôpital Henri Mondor, Créteil, France | en_US |
dc.identifier.pmid | 1606170 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29986/1/0000351.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(92)90223-I | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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