Pulmonary endothelial cell ATP depletion following intestinal ischemia
dc.contributor.author | Gerkin, Todd M. | en_US |
dc.contributor.author | Welling, Theodore H. | en_US |
dc.contributor.author | Turnage, Richard H. | en_US |
dc.contributor.author | Ryan, Una S. | en_US |
dc.contributor.author | Guice, Karen S. | en_US |
dc.contributor.author | Oldham, Keith T. | en_US |
dc.date.accessioned | 2006-04-10T15:12:13Z | |
dc.date.available | 2006-04-10T15:12:13Z | |
dc.date.issued | 1992-06 | en_US |
dc.identifier.citation | Gerkin, Todd M., Welling, Theodore H., Turnage, Richard H., Ryan, Una S., Guice, Karen S., Oldham, Keith T. (1992/06)."Pulmonary endothelial cell ATP depletion following intestinal ischemia." Journal of Surgical Research 52(6): 642-647. <http://hdl.handle.net/2027.42/30022> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-4BNF371-29P/2/ffb3741348856e29100d887405d3b869 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30022 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1528042&dopt=citation | en_US |
dc.description.abstract | Multiple organ failure (MOF) is known to follow systemic inflammatory mediator activation associated with intestinal ischemia--reperfusion injury. In particular, the pulmonary microvasculature appears to be susceptible to MOF-related injury. This study was designed to evaluate the hypothesis that non-cellular plasma factors associated with intestinal ischemia without reperfusion also mediate pulmonary endothelial cell injury. Male Sprague--Dawley rats had intestinal ischemia induced by microvascular clip occlusion of the superior mesenteric artery for 30, 60, 90, or 120 min. Following each period of ischemia, plasma samples were obtained from the portal vein. Time-matched sham-operated animals served as controls. Monolayers of cultured rat pulmonary artery endothelial cells were then incubated with the plasma samples and ATP levels determined using a luciferin--luciferase assay. A 51Cr-release assay using labeled endothelial cells was performed under identical conditions to assess cytotoxicity. Endothelial cell ATP levels were 1.99 +/- 0.23 x 10-11 mole/[mu]g DNA in sham preparations. After a 4-hr incubation in plasma from the 90 and 120 min ischemia groups, cellular ATP levels fell significantly to 1.07 +/- 0.23 x 10-11 mole/[mu]g DNA, respectively (P P = NS). All animals survived 24 hr in the sham, 30, and 60 min groups. However, survival was 40 and 0% in the 90 and 120 min groups, respectively (P in vitro data with in vivo survival. | en_US |
dc.format.extent | 833216 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Pulmonary endothelial cell ATP depletion following intestinal ischemia | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Sections of General Surgery and Pediatric Surgery, Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Sections of General Surgery and Pediatric Surgery, Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Sections of General Surgery and Pediatric Surgery, Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Sections of General Surgery and Pediatric Surgery, Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Division of General and Thoracic Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA; Sections of General Surgery and Pediatric Surgery, Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.contributor.affiliationother | Division of Pediatric Surgery, Department of Surgery, Duke University of Medical Center, Durham, North Carolina 27710, USA | en_US |
dc.identifier.pmid | 1528042 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30022/1/0000390.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-4804(92)90143-N | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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