Cell cycle progression is associated with distinct patterns of phosphorylation of Op18
dc.contributor.author | Strahler, John R. | en_US |
dc.contributor.author | Lamb, Barbara J. | en_US |
dc.contributor.author | Ungar, David R. | en_US |
dc.contributor.author | Fox, David A. | en_US |
dc.contributor.author | Hanash, Samir M. | en_US |
dc.date.accessioned | 2006-04-10T15:12:50Z | |
dc.date.available | 2006-04-10T15:12:50Z | |
dc.date.issued | 1992-05-29 | en_US |
dc.identifier.citation | Strahler, John R., Lamb, Barbara J., Ungar, David R., Fox, David A., Hanash, Samir M. (1992/05/29)."Cell cycle progression is associated with distinct patterns of phosphorylation of Op18." Biochemical and Biophysical Research Communications 185(1): 197-203. <http://hdl.handle.net/2027.42/30037> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4FW0C1P-7V/2/d5e3e6db94694ac85eb69cef407555c1 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30037 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1376116&dopt=citation | en_US |
dc.description.abstract | Op18 is a highly conserved major cytosolic phosphoprotein which has been implicated in signal transduction in a wide variety of cell types. Freshly isolated peripheral blood lymphocytes (PBL) constitutively express low levels of mostly unphosphorylated Op18. Following mitogenic stimulation of PBL, Op18 synthesis is induced at a time when cells are entering S-phase. In this study we have characterized Op18 phosphorylation during progression of freshly isolated PBL through the cell cycle. Transition from G0 to G1 following activation with OKT3 was associated with an increase in a phosphorylated form designated Op18c. Progression of cells through G1 into S resulted in an increase in phosphorylated Op18 forms, designated Op18a and Op18b, which paralleled new Op18 synthesis. Transition of cells into G2+M resulted in the appearance of the more acidic phosphorylated forms Op18d and Op18e. Calphostin C, a specific inhibitor of protein kinase C, dramatically decreased all forms of phosphorylated Op18 in OKT3 treated Jurkat cells. Our results suggest that Op18 phosphorylation is mediated in part by PKC activation as well as by other kinases yielding different phosphorylated forms at specific stages of the cell cycle. | en_US |
dc.format.extent | 2985452 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cell cycle progression is associated with distinct patterns of phosphorylation of Op18 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Department of Pediatrics, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Department of Pediatrics, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Department of Pediatrics, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Department of Internal Medicine, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Department of Pediatrics, Ann Arbor, MI, 48109, USA | en_US |
dc.identifier.pmid | 1376116 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30037/1/0000405.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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