Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase
dc.contributor.author | Powell, Steven M. | en_US |
dc.contributor.author | Zalkin, Howard | en_US |
dc.contributor.author | Dixon, Jack E. | en_US |
dc.date.accessioned | 2006-04-10T15:12:57Z | |
dc.date.available | 2006-04-10T15:12:57Z | |
dc.date.issued | 1992-05-25 | en_US |
dc.identifier.citation | Powell, Steven M., Zalkin, Howard, Dixon, Jack E. (1992/05/25)."Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase." FEBS Letters 303(1): 4-10. <http://hdl.handle.net/2027.42/30040> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T36-44XN10B-C5/2/8075d1dc19d35b4dbf822862fc4b41aa | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30040 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1592113&dopt=citation | en_US |
dc.description.abstract | Adenylosuccinate synthetase (AS) catalyzes the first committed step in the conversion of IMP to AMP. A cDNA was isolated from a human liver library which encodes a protein of 455 amino acids (Mr of 49.925). Alignments of human, mouse, Dictyostelium discoideum and E. coli AS sequences identify a number of invariant residues which are likely to be important for structure and/or catalysis. The human AS sequence was also 19% identical to the human urea cycle enzyme, arginosuccinate synthetase (ASS), which catalyses a chemically similar reaction. Both human liver and HcLa AS mRNA showed signals of 2.3 and 2.8 kb. An unmodified N-terminus is required for function of the human AS enzyme in E. coli mutants lacking the bacterial enzyme. The human cDNA provides a means to assess the possible role of AS abnormalities in unclassified, idiopathic cases of gout. | en_US |
dc.format.extent | 729873 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Purdue University, West Lafayette, IN 49707, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Purdue University, West Lafayette, IN 49707, USA | en_US |
dc.identifier.pmid | 1592113 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30040/1/0000408.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-5793(92)80465-S | en_US |
dc.identifier.source | FEBS Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.