Interaction of linear manno-oligosaccharides with three mannose-specific bulb lectins. Comparison with mannose/glucose-binding lectins
dc.contributor.author | Kaku, Hanae | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-10T15:13:05Z | |
dc.date.available | 2006-04-10T15:13:05Z | |
dc.date.issued | 1992-05-22 | en_US |
dc.identifier.citation | Kaku, Hanae, Goldstein, Irwin J. (1992/05/22)."Interaction of linear manno-oligosaccharides with three mannose-specific bulb lectins. Comparison with mannose/glucose-binding lectins." Carbohydrate Research 229(2): 337-346. <http://hdl.handle.net/2027.42/30043> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TFF-42NR3XP-99/2/6c6aed1cad3494dbbb79e74d02d009be | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30043 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1394290&dopt=citation | en_US |
dc.description.abstract | Three new mannose-binding lectins, isolated from daffodil (NPA), amaryllis (HHA), and snowdrop (GNA) bulbs, are capable of precipitating with a linear mannopentaose (Man[alpha]1-3Man[alpha]1-3Man[alpha]1-3Man[alpha]1-2Man). NPA and HHA reacted strongly with the mannopentaose whereas GNA gave a precipitate only at concentrations> 500 [mu]M. A phosphate group at C-6 of the nonreducing terminal mannosyl group prevented precipitation in all three cases. The reduced (NaBH4) mannopentaose, Man4Man-ol, did not precipitate with GNA or NPA, but was active with HHA. This activity was lost when Man4Man-ol was converted (NaIO4 then NaBH4; mild acid hydrolysis of the reduced product) into trisaccharide derivatives. With [alpha]--Manp-OMe the three lectins gave UV difference spectra having large positive peaks at 292-293 and 283-284 nm, and a small positive peak at 275 nm, characteristic of tryptophanyl and tyrosyl residues. The association constants for the interaction with [alpha]--Manp-OMe were very low (NPA, 86; HHA, 66; and GNA, 41 M-1), but the lectins bound methyl (1 --> 3)-[alpha]-mannobioside with increased affinity (K for NPA 540, for HHA 2400, and for GNA 200 M-1). The bulb lectins lack binding sites for hydrophobic ligands, as judged by their failure to interact with the fluorescent probes 8-anilino-1-naphthalensulfonic acid (ANS) and 6-p-toluidino-2-naphthalenesulfonic acid (TNS). | en_US |
dc.format.extent | 809152 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Interaction of linear manno-oligosaccharides with three mannose-specific bulb lectins. Comparison with mannose/glucose-binding lectins | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 USA | en_US |
dc.identifier.pmid | 1394290 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30043/1/0000411.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0008-6215(00)90579-2 | en_US |
dc.identifier.source | Carbohydrate Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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