High-resolution analysis of c-fos chromatin accessibility using a novel DNase I-PCR assay
dc.contributor.author | Feng, Junli | en_US |
dc.contributor.author | Villeponteau, Bryant | en_US |
dc.date.accessioned | 2006-04-10T15:15:50Z | |
dc.date.available | 2006-04-10T15:15:50Z | |
dc.date.issued | 1992-04-06 | en_US |
dc.identifier.citation | Feng, Junli, Villeponteau, Bryant (1992/04/06)."High-resolution analysis of c-fos chromatin accessibility using a novel DNase I-PCR assay." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1130(3): 253-258. <http://hdl.handle.net/2027.42/30107> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1V-488932C-3N/2/b3bcfb6ab5dc657cf1562c09e123225f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30107 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1562603&dopt=citation | en_US |
dc.description.abstract | In our previous study, c-fos chromatin accessibility was assayed using DNase I digestion and Southern blot analysis. This low-resolution mapping of c-fos chromatin accessibility demonstrated that serum stimulation of the c-fos enhancer induces a reversivle increase in c-fos DNase I sensitivity and suggested that a 5' to 3' gradient of DNase I sensitivity may form downstream from the c-fos enhancer. To confirm the existence of a 5' to 3' gradient of accessibility, we have recently developed a high-resolution polymerase chain reaction (PCR) assay for DNase I sensitivity. Using this novel DNase I assay, we have reliably detected position- and time-dependent gradients of chromatin accessibility around the c-fos enhancer. These data confirm our earlier results and further support the hypothesis that the changes in c-fos chromatin accessibility originate near the 5' enhancer. As a technique for future examinations of gene structure, our data demonstrate the value of the DNase I-PCR assay for rapidly preparing comprehensive and high-resolution maps of chromatin accessibility for any sequenced genomic region. | en_US |
dc.format.extent | 614551 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | High-resolution analysis of c-fos chromatin accessibility using a novel DNase I-PCR assay | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Institute of Gerontology, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA; The Institute of Gerontology, University of Michigan, Ann Arbor, MI, USA. | en_US |
dc.identifier.pmid | 1562603 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30107/1/0000479.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4781(92)90437-5 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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