Unimpaired formation of hormone-stimulated inositol triphosphate in human mesangial cells under hyperglycemic conditions
dc.contributor.author | Greenberg, Lawrence | en_US |
dc.contributor.author | Urbanes, Aris Q. | en_US |
dc.contributor.author | Shayman, James A. | en_US |
dc.date.accessioned | 2006-04-10T15:17:31Z | |
dc.date.available | 2006-04-10T15:17:31Z | |
dc.date.issued | 1992-03-20 | en_US |
dc.identifier.citation | Greenberg, Lawrence, Urbanes, Aris Q., Shayman, James A. (1992/03/20)."Unimpaired formation of hormone-stimulated inositol triphosphate in human mesangial cells under hyperglycemic conditions." Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1138(3): 229-235. <http://hdl.handle.net/2027.42/30148> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1Y-47NVY1K-7P/2/0d2b1e3bae7ef2e4ba7c2433f9490c6f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30148 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1312360&dopt=citation | en_US |
dc.description.abstract | The relationship between bulk cellular myo-inositol content and phosphatidylinositol metabolism was evaluated in a human mesangial cell line under euglycemic and hyperglycemic conditions. Mesangial cells maintained in high glucose medium displayed a concentration-dependent fall in myo-inositol as measured by gas-liquid chromatography. Measurements of phosphatidylinositol, phosphatidylinositol 4-monophosphate and phosphatidylinositol 4,5-biphosphate mass revealed slight but statistically insignificant increases in cells exposed to high glucose containing medium. CDP-diacylglycerol: myo-inositol 3-phosphatidylinositol transferase activity, measured in plasma membranes from mesangial cells grwon under control and hyperglycemic conditions, was kinetically similar with Michaelis constants (Km values) for myo-inositol of 2.9 and 2.1 mM, respectively. Finally hormone-stimulated intracellular calcium mobilization and myo-inositol 1,4,5-triphosphate mass was measured from mesangial cells grown under normal and hyperglycemic conditions. Both intracellular calcium and inositol triphosphate formation were unchanged in cells previously exposed to high glucose conditions (400 mg/dl) compared to cells grown under normal glucose concentration (100 mg/dl). These data indicate that bulk changes in myo-inositol induced by hyperglycemia are neither associated with alterations in basal levels of inositol containing glycerolipids nor with changes in hormone-stimulated calcium mobilization and inositol trisphosphate formation under conditions of short term changes in extracellular glucose. | en_US |
dc.format.extent | 682222 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Unimpaired formation of hormone-stimulated inositol triphosphate in human mesangial cells under hyperglycemic conditions | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Neophrology, University of Michigan and Veterans Administration Medical Centers, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Neophrology, University of Michigan and Veterans Administration Medical Centers, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Neophrology, University of Michigan and Veterans Administration Medical Centers, Ann Arbor, MI, U.S.A. | en_US |
dc.identifier.pmid | 1312360 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30148/1/0000525.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0925-4439(92)90042-L | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.