Subset-specific co-stimulatory signals are required for IL-2 production but not growth inhibition responses by T cell hybrids specific for myelin basic protein
dc.contributor.author | Mannie, M. D. | en_US |
dc.contributor.author | Nairn, Roderick | en_US |
dc.date.accessioned | 2006-04-10T15:18:46Z | |
dc.date.available | 2006-04-10T15:18:46Z | |
dc.date.issued | 1992-03 | en_US |
dc.identifier.citation | Mannie, M. D., Nairn, R. (1992/03)."Subset-specific co-stimulatory signals are required for IL-2 production but not growth inhibition responses by T cell hybrids specific for myelin basic protein." Cellular Immunology 140(1): 219-236. <http://hdl.handle.net/2027.42/30179> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCF-4DKSWDK-68/2/fbc7d065321f91398ce1d5ae90bf212d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30179 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1371244&dopt=citation | en_US |
dc.description.abstract | Two distinct types of T cell hybridomas (designated THYB-1 and THYB-2) were derived by fusing BW5147 thymoma cells with encephalitogenic T helper cells from Lewis rats. Both subsets required MHC-restricted presentation of determinants within the 72-86 peptide sequence of myelin basic protein (MBP) as a requisite signal for IL-2 production. Unlike THYB-1 hybrids, however, THYB-2 hybrids required additional accessory cell activities that were mediated by radiosensitive nonadherent (RS-NAdh) splenocytes (SPL). In this study, we describe two observations indicating that RS-NAdh SPL enable MBP-specific responses of THYB-2 hybrids by providing subset-specific co-stimulatory signals that act independently of antigen recognition pathways. First, RS-NAdh SPL were required by THYB-2 hybrids for MBP-stimulated IL-2 production but were not needed when MBP-specific inhibition of hybrid growth was used as an alternative measure of cellular activation. Second, PMA and ionomycin induced optimal IL-2 production by both tHYB-1 hybrids and BW5147 thymoma cells but only stimulated low or marginal levels of IL-2 production by THYB-2 hybrids. Together, these observations indicate that RS-NAdh SPL were required for the specific response of IL-2 production regardless of whether the response was stimulated by antigen or by mitogens that bypass initial antigen recognition events. This study thereby provides additional evidence that distinct stimulus-response relationships define two T-helper cell lineages in experimental autoimmune encephalomyelitis. | en_US |
dc.format.extent | 1263797 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Subset-specific co-stimulatory signals are required for IL-2 production but not growth inhibition responses by T cell hybrids specific for myelin basic protein | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0620, U.S.A.; Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0620, U.S.A. | en_US |
dc.identifier.pmid | 1371244 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30179/1/0000564.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0008-8749(92)90189-V | en_US |
dc.identifier.source | Cellular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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