Alu-PCR: Characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers
dc.contributor.author | Meese, Eckart U. | en_US |
dc.contributor.author | Meltzer, Paul S. | en_US |
dc.contributor.author | Ferguson, Paul W. | en_US |
dc.contributor.author | Trent, Jeffrey M. | en_US |
dc.date.accessioned | 2006-04-10T15:19:25Z | |
dc.date.available | 2006-04-10T15:19:25Z | |
dc.date.issued | 1992-03 | en_US |
dc.identifier.citation | Meese, Eckart U., Meltzer, Paul S., Ferguson, Paul W., Trent, Jeffrey M. (1992/03)."Alu-PCR: Characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers." Genomics 12(3): 549-554. <http://hdl.handle.net/2027.42/30195> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-4DYM8X6-9X/2/ce9fdd98d26f4c85e72a54ee4de648ac | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30195 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1559706&dopt=citation | en_US |
dc.description.abstract | The Alu-polymerase chain reaction (Alu-PCR) was applied to selectively amplify DNA sequences from human chromosome 6 using a single primer (A1) directed to the human Alu consensus sequence. A specific amplification pattern was demonstrated for a panel of eight somatic cell hybrids containing different portions of chromosome 6. This PCR pattern permits the identification of submicroscopic DNA alterations and can be utilized as a reference for additional chromosome 6-specific hybrids. To obtain new chromosome 6-specific markers we established two libraries from PCR-amplified sequences using two somatic cell hybrids (MCH381.2D and 640-5A). Out of a total of 109 clones that were found to be chromosome 6 specific, 13 clones were regionally assigned. We also included a procedure that allows the isolation of chromosome 6-specific markers from hybrids that contain human chromosomes other than 6. Our results will contribute to the molecular characterization of chromosome 6 by fostering characterization of somatic cell hybrids and by the generation of new regicnally assigned DNA markers. | en_US |
dc.format.extent | 2616068 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Alu-PCR: Characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical Center Department of Radiation Oncology MSRBII C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical Center Department of Pediatrics, MSRBII C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical Center Department of Radiation Oncology MSRBII C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical Center Department of Human Genetics, MSRBII C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA; University of Michigan Medical Center Department of Radiation Oncology MSRBII C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA. | en_US |
dc.identifier.pmid | 1559706 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30195/1/0000583.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0888-7543(92)90447-Z | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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