Show simple item record

Association of the transformed glucocorticoid receptor with a cytoskeletal protein complex

dc.contributor.authorScherrer, Lawrence C.en_US
dc.contributor.authorPratt, William B.en_US
dc.date.accessioned2006-04-10T15:19:35Z
dc.date.available2006-04-10T15:19:35Z
dc.date.issued1992-03en_US
dc.identifier.citationScherrer, Lawrence C., Pratt, William B. (1992/03)."Association of the transformed glucocorticoid receptor with a cytoskeletal protein complex." The Journal of Steroid Biochemistry and Molecular Biology 41(3-8): 719-721. <http://hdl.handle.net/2027.42/30199>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T8X-47DTCF8-2D/2/43d5b1e27d7d39ca5dffd6a7f3efa930en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/30199
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1562545&dopt=citationen_US
dc.description.abstractIn a recent paper we described a system in which glucocorticoid receptors associate with particulate complexes containing tubulin [Cancer Res. 49 (1989) 2222s-2229s]. When L cell cytosol is mixed with a microtubule stabilizing buffer and heated to 37[deg]C, the receptor becomes associated with a complex that can be centrifuged out of solution at 150,000 g. In this work we show that the glucocorticoid receptor--cytoskeletal protein complex forms in a temperature and glutamate-dependent manner. Molybdate does not affect generation of the cytoskeletal protein complex but it inhibits association of the receptor with the complex. This suggests that transformation of the receptor to its DNA-binding form is required for interaction with the cytoskeletal complex. Colchicine has no effect on generation of the particulate complex or on the association of receptor with it, suggesting that formation of the complex does not represent a classic in vitro process of tubulin polymerization.en_US
dc.format.extent363798 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleAssociation of the transformed glucocorticoid receptor with a cytoskeletal protein complexen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.identifier.pmid1562545en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/30199/1/0000587.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0960-0760(92)90411-Ben_US
dc.identifier.sourceThe Journal of Steroid Biochemistry and Molecular Biologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.