A ceramide analogue (PDMP) inhibits glycolipid synthesis in fish embryos
dc.contributor.author | Fenderson, Bruce A. | en_US |
dc.contributor.author | Ostrander, Gary K. | en_US |
dc.contributor.author | Hausken, Zach | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.contributor.author | Hakomori, Sen-itiroh | en_US |
dc.date.accessioned | 2006-04-10T15:20:58Z | |
dc.date.available | 2006-04-10T15:20:58Z | |
dc.date.issued | 1992-02 | en_US |
dc.identifier.citation | Fenderson, Bruce A., Ostrander, Gary K., Hausken, Zach, Radin, Norman S., Hakomori, Sen-itiroh (1992/02)."A ceramide analogue (PDMP) inhibits glycolipid synthesis in fish embryos." Experimental Cell Research 198(2): 362-366. <http://hdl.handle.net/2027.42/30233> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFC-4DKKFT3-6N/2/ac7d166f2ede408596a8a4f33f496074 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30233 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1729139&dopt=citation | en_US |
dc.description.abstract | Glycolipids were depleted from medaka embryos using 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an inhibitor of glucosylceramide synthetase. Embryos cultured in the presence of 20 [mu]M PDMP exhibited a dramatic decline in glycolipid synthesis and cell surface expression. Metabolic labeling of glucosylceramide declined by 87% on Days 3-6 of development and 72% on Days 7-10 (hatching occurred on Day 10). In parallel, PDMP-treated embryos exhibited a striking loss of several tissue-specific glycolipid antigens, including 9-O-acetyl GD3 from brain and retina, GT3/ GQ1C from brain, neural tube, and retina, and sulfated glycolipid from skin and gut. Despite these changes in glycolipid expression, PDMP-treated embryos were fully viable with no evidence of developmental abnormality. PDMP appears to provide a useful tool for identifying glycolipid antigens in embryos and investigating their role in development. | en_US |
dc.format.extent | 3510277 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A ceramide analogue (PDMP) inhibits glycolipid synthesis in fish embryos | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, 1103 East Huron, University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationother | The Biomembrane Institute, 201 Elliott Avenue West, Seattle, Washington 98119, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Zoology, LSW 430, Oklahoma State University, Stillwater, Oklahoma 74078, U.S.A. | en_US |
dc.contributor.affiliationother | The Biomembrane Institute, 201 Elliott Avenue West, Seattle, Washington 98119, U.S.A. | en_US |
dc.contributor.affiliationother | The Biomembrane Institute, 201 Elliott Avenue West, Seattle, Washington 98119, U.S.A. | en_US |
dc.identifier.pmid | 1729139 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30233/1/0000627.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-4827(92)90392-L | en_US |
dc.identifier.source | Experimental Cell Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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