Specific G proteins mediate endothelin induced contraction

Abstract

Endothelin is a potent vasoconstrictor peptide which has recently been localized in the gastrointestinal tract. We have investigated the transmembrane signaling properties of endothelin in isolated smooth muscle cells of the rabbit rectosigmoid. Endothelin induced a dose dependent contraction of smooth muscle cells in a range of 10-10 to 10-6M. In normal buffer, contraction peaked at 30 sec and was sustained for up to 8 min. Incubation in 0Ca/2mM EGTA abolished the sustained contraction induced by endothelin, but had no effect on the initial transient contraction. Preincubation of saponin treated cells with G protein antisera had no effect on control cell length. Preincubation of saponin treated isolated smooth muscle cells with specific G protein antisera (rabbit antisera) for Go[alpha] or Gs for 60 minutes did not inhibit contraction induced by endothelin. Preincubation with an antiserum to Gi3[alpha] inhibited the initial transient contraction induced by endothelin and preincubation with an antiserum to Gi1-2[alpha] inhibited the sustained phase of the endothelin induced contraction. Our data indicate that: 1) Endothelin induces a direct sustained contraction of smooth cells from the rectosigmoid; 2) The transmembrane signalling of endothelin is through two specific GTP binding components that are Gi[alpha], one for the initial transient contraction, and the other for the sustained phase of the contraction.