Determination of plasma propofol levels using gas chromatography--mass spectrometry with selected-ion monitoring
dc.contributor.author | Stetson, Phillip L. | en_US |
dc.contributor.author | Domino, Edward F. | en_US |
dc.contributor.author | Sneyd, J. R. | en_US |
dc.date.accessioned | 2006-04-10T15:32:55Z | |
dc.date.available | 2006-04-10T15:32:55Z | |
dc.date.issued | 1993-10-29 | en_US |
dc.identifier.citation | Stetson, P. L., Domino, E. F., Sneyd, J. R. (1993/10/29)."Determination of plasma propofol levels using gas chromatography--mass spectrometry with selected-ion monitoring." Journal of Chromatography: Biomedical Applications 620(2): 260-267. <http://hdl.handle.net/2027.42/30507> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG9-457CTXX-CW/2/3b8a3524933256d4547df3a00f4e134d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30507 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8300796&dopt=citation | en_US |
dc.description.abstract | Propofol (2,6-diisopropylphenol, I.C.I. 35 868) is a rapid-acting, intravenous anesthetic agent recently introduced for the induction and maintenance of general anesthesia. This paper describes a gas chromatographic--mass spectrometric procedure using selected-ion monitoring for the determination of plasma propofol levels. The drug and the internal standard (thymol) were extracted from plasma into diethyl ether--pentane, and derivatized to their trimethylsilyl derivatives before analysis. The reproducibility of the daily standard curves had coefficients of variation ranging from 2.7% to 10.2%. The precision of the assay yielded a coefficient of variation ranging from 4.5% to 5.6%, and the concentration means for the seeded control samples were found to be within -1.6% to +0.6% of the theoretical values for propofol. No interfering peaks have been observed in application of this procedure to either normal volunteer or patient samples. The minimum detectable level under the conditions described was 0.20 ng propofol/ml plasma. This assay and a high-performance liquid chromatographic assay with fluorescence detection were both used to measure plasma propofol concentrations in 89 human plasma samples, and the correlation between the two methods was excellent. | en_US |
dc.format.extent | 753683 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Determination of plasma propofol levels using gas chromatography--mass spectrometry with selected-ion monitoring | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, Upjohn Center for Clinical Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, MI 48109 USA | en_US |
dc.identifier.pmid | 8300796 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30507/1/0000136.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-4347(93)80014-U | en_US |
dc.identifier.source | Journal of Chromatography: Biomedical Applications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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