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Quantitative Analysis of Changes in the Molecular Species of Glycerolipids in Cultured Cells during Signal Transduction
dc.contributor.authorLee Chunghee,en_US
dc.contributor.authorHajra Amiya K. ,en_US
dc.date.accessioned2006-04-10T15:33:46Z
dc.date.available2006-04-10T15:33:46Z
dc.date.issued1993-10en_US
dc.identifier.citationLee Chunghee, , Hajra Amiya K., (1993/10)."Quantitative Analysis of Changes in the Molecular Species of Glycerolipids in Cultured Cells during Signal Transduction." Neuroprotocols 3(2): 83-90. <http://hdl.handle.net/2027.42/30528>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WNS-45P69GF-H/2/de052e0a39dcebf3c35d44059cd5cb11en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/30528
dc.description.abstractA method for the quantitative analysis of the molecular species of glycerolipids present In biological samples has been described. 1,2-Diacyl-sn-glycerol, either isolated from biological samples or enzymatically generated from phosphoglycerides, is benzoylated at the sn-3 position and then subjected to reverse-phase (C18-silica) HPLC to separate the molecular species of different hydrophobicitles. An internal standard (1,2-distearoyl-sn-glycerol) is used to identify and quantify the various species eluted from the reverse-phase column. Examples are given for the quantitative analysis of molecular species and precursor-product relationships of glycerolipids generated In SK-N-SH neuroblastoma cells after stimulation of the cell-surface muscarinic acetylcholine receptors.en_US
dc.format.extent684942 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleQuantitative Analysis of Changes in the Molecular Species of Glycerolipids in Cultured Cells during Signal Transductionen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumNeuroscience Laboratory, Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104-1687en_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104-1687en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/30528/1/0000160.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1006/ncmn.1993.1041en_US
dc.identifier.sourceNeuroprotocolsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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