Muscarinic modulation of conductances underlying the afterhyperpolarization in neurons of the rat basolateral amygdala
dc.contributor.author | Womble, Mark D. | en_US |
dc.contributor.author | Moises, Hylan C. | en_US |
dc.date.accessioned | 2006-04-10T15:35:47Z | |
dc.date.available | 2006-04-10T15:35:47Z | |
dc.date.issued | 1993-09-03 | en_US |
dc.identifier.citation | Womble, Mark D., Moises, Hylan C. (1993/09/03)."Muscarinic modulation of conductances underlying the afterhyperpolarization in neurons of the rat basolateral amygdala." Brain Research 621(1): 87-96. <http://hdl.handle.net/2027.42/30577> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-48361GT-2CK/2/873db39a76d4c8496ea230c016dae26c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30577 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8221077&dopt=citation | en_US |
dc.description.abstract | The excitability level of pyramidal neurons in the basolateral amygdala (BLA) is greatly increased following muscarinic receptor activation, an effect associated with an increased rate of action potential firing and reduction of the afterhyperpolarization (AHP). We impaled BLA pyramidal neurons in slices of rat ventral forebrain with a single microelectrode to examine the currents underlying the AHP and spike frequency accomodation and determine their sensitivities to muscarinic modulation. In voltage-clamp, depolarizing steps were followed by biphasic outward tail currents, consisting of rapidly decaying (IFast) and slowly decaying (ISlow) current components. These corresponded temporally with the medium and slow portions of the AHP, respectively. The reversal potential or the IFast component of the AHP tail current shifted in the depolarizing direction with increases in the extracellular K+ concentration. The amplitude of IFast was reduced during perfusion of 0-Ca2+ medium or by superfusion of TEA (1-5 mM) or carbachol (10-40 [mu]M). It is suggested that IFast was produced by the rapidly decaying Ca2+-activated K+ current (IC) and the muscarinic-sensitive M-current (IM). The ISlow tail current component reversed at the estimated values for EK in medium containing either normal or elevated K+ levels. This component was eliminated by perfusion of 0-Ca2+ medium or inclusion of cyclic-AMP in the recording electrode. It was not blocked by TEA (5 mM) or apamin (50-500 nM), but was reduced by carbachol in a dose-dependent manner (IC50=0.5 [mu]M). Electrical stimulation cholinergic afferent pathways to the BLA produced inhibition of ISlow, an effect which was enhanced by eserine and prevented by atropine. Loss of the ISlow component was always accompanied by similar reductions in accomodation and the slow AHP. It was concluded that this tail current component resulted from the slowly decaying Ca2+-activated K+ current, IAHP. Thus, the muscarinic inhibition of IAHP contributes to the enhanced excitability exhibited by BLA pyramidal neurons following cholinergic stimulation. | en_US |
dc.format.extent | 1046941 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Muscarinic modulation of conductances underlying the afterhyperpolarization in neurons of the rat basolateral amygdala | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physiology, The University of Michigan Medical School, Ann Arbor, MI 49109-0622, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, The University of Michigan Medical School, Ann Arbor, MI 49109-0622, USA | en_US |
dc.identifier.pmid | 8221077 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30577/1/0000212.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(93)90301-3 | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.