An Anti-CD2 Monoclonal Antibody That Both Inhibits and Stimulates T Cell Activation Recognizes a Subregion of CD2 Distinct from Known Ligand-Binding Sites
dc.contributor.author | Kozarsky, Karen F. | en_US |
dc.contributor.author | Tsai, Carlene | en_US |
dc.contributor.author | Bott, Cynthia M. | en_US |
dc.contributor.author | Allada, Gopal | en_US |
dc.contributor.author | Li, Lan Lan | en_US |
dc.contributor.author | Fox, David A. | en_US |
dc.date.accessioned | 2006-04-10T15:36:43Z | |
dc.date.available | 2006-04-10T15:36:43Z | |
dc.date.issued | 1993-09 | en_US |
dc.identifier.citation | Kozarsky, Karen F., Tsai, Carlene, Bott, Cynthia M., Allada, Gopal, Li, Lan Lan, Fox, David A. (1993/09)."An Anti-CD2 Monoclonal Antibody That Both Inhibits and Stimulates T Cell Activation Recognizes a Subregion of CD2 Distinct from Known Ligand-Binding Sites." Cellular Immunology 150(2): 235-246. <http://hdl.handle.net/2027.42/30600> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCF-45R7930-6C/2/04fa97347db319af3495d8cc573e6c89 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30600 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8103706&dopt=citation | en_US |
dc.description.abstract | The T lymphocyte glycoprotein CD2 appears to have an important role in human T cell development and activation. A novel anti-CD2 monoclonal antibody, designated UMCD2, was shown to block E rosetting, and therefore was defined as recognizing the T111 ligand-binding epitope. Binding of UMCD2 to T cells and thymocytes was blocked by several, but not all, anti-T111 antibodies, suggesting that the T111 eptope consists of more than one subepitope. In functional studies, the combination of UMCD2 plus anti-T113 was mitogenic for T cells; in some individuals, the level of activation was as high as that seen for the combination of anti-T112 plus anti-T113. However, when UMCD2 was added to other stimuli mitogenic for T lymphocytes, such as IL-2 or anti-CD3-Sepharose, it inhibited T cell responses. Although the combination of UMCD2 and anti-T113 induced an increase in cytoplasmic free calcium, the inhibitory activities of UMCD2 were not accompanied by effects on calcium fluxes. A panel of previously characterized CD2 mutants was then analyzed for binding of UMCD2 and other anti-CD2 monoclonals. Surprisingly, UMCD2 bound to all mutants tested, although the other anti-CD2 antibodies with specificity for the ligand-binding region of CD2 each failed to bind to one or more mutants. These data suggest that binding of antibody to a particular CD2 epitope can have opposite effects on the state of T cell activation, depending on the costimulus. Moreover, inhibitory effects mediated through CD2 may use a signaling mechanism distinct from that used in CD2 pathway activation. Of particular interest, the portion of the CD2 ligand-binding region recognized by UMCD2 is distinct from areas of the CD2 molecule that have previously been studied. | en_US |
dc.format.extent | 409982 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | An Anti-CD2 Monoclonal Antibody That Both Inhibits and Stimulates T Cell Activation Recognizes a Subregion of CD2 Distinct from Known Ligand-Binding Sites | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, Rackham Arthritis Research Unit and the Multipurpose Arthritis Center, University of Michigan Medical Center, 3918 Taubman Center, Box 0358, Ann Arbor, Michigan 48109-0358 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, Rackham Arthritis Research Unit and the Multipurpose Arthritis Center, University of Michigan Medical Center, 3918 Taubman Center, Box 0358, Ann Arbor, Michigan 48109-0358 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, Rackham Arthritis Research Unit and the Multipurpose Arthritis Center, University of Michigan Medical Center, 3918 Taubman Center, Box 0358, Ann Arbor, Michigan 48109-0358 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, Rackham Arthritis Research Unit and the Multipurpose Arthritis Center, University of Michigan Medical Center, 3918 Taubman Center, Box 0358, Ann Arbor, Michigan 48109-0358 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, Rackham Arthritis Research Unit and the Multipurpose Arthritis Center, University of Michigan Medical Center, 3918 Taubman Center, Box 0358, Ann Arbor, Michigan 48109-0358 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, Rackham Arthritis Research Unit and the Multipurpose Arthritis Center, University of Michigan Medical Center, 3918 Taubman Center, Box 0358, Ann Arbor, Michigan 48109-0358 | en_US |
dc.identifier.pmid | 8103706 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30600/1/0000237.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/cimm.1993.1193 | en_US |
dc.identifier.source | Cellular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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