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Substance P excites neurons in the gustatory zone of the rat nucleus tractus solitarius

dc.contributor.authorKing, Michael S.en_US
dc.contributor.authorWang, Limeien_US
dc.contributor.authorBradley, Robert M.en_US
dc.date.accessioned2006-04-10T15:38:33Z
dc.date.available2006-04-10T15:38:33Z
dc.date.issued1993-08-13en_US
dc.identifier.citationKing, Michael S., Wang, Limei, Bradley, Robert M. (1993/08/13)."Substance P excites neurons in the gustatory zone of the rat nucleus tractus solitarius." Brain Research 619(1-2): 120-130. <http://hdl.handle.net/2027.42/30639>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6SYR-482YW6R-7T/2/9018c16d5e861b5cb6364af2a3355d4den_US
dc.identifier.urihttps://hdl.handle.net/2027.42/30639
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7690670&dopt=citationen_US
dc.description.abstractWhole-cell patch recordings of neurons in the rostal (gustatory) nucleus tractus solitarius (rNTS) were performed in a brain slice preparation from rat medulla. Neural responses to brief applications (10-45 s) of substance P (SP), via a constant superfusion apparatus, were recorded. SP transiently depolarized 80 of 117 (68%) rNTS neurons in a dose-dependent manner. Sub-micromolar concentrations of SP had potent excitatory effects, and the half maximal response occurred at 0.6 [mu]M. The depolarizing effect of SP was accompanied by an increase in input resistance in 81% of the responsive neurons. The excitatory effects of SP persisted in low Ca2+ (0.2 mM) and high Mg2+ (12 mM) saline as well as in the presence of 2 [mu]M TTX (n = 5 for each), suggesting direct postsynaptic action on the recorded neurons. SP also hyperpolarized 4 neurons (4%) and had no effect on 33 neurons (28%). Each of the 4 neurons which were hyperpolarized by SP showed a decrease in input resistance. A more detailed assessment of the types of neurons in the rNTS which respond to SP was also conducted. Neurons were separated into 4 electrophysiological groups on the basis of their repetitive firing pattern induced by a hyperpolarizing and depolarizing current injection paradigm. Neurons belonging to each of the 4 electrophysiological groups responded to SP. Eighteen neurons, which were filled with 1% biocytin during recording, were categorized as ovoid, multipolar or fusiform based on their morphological characteristics. SP excited all 3 morphological types of neurons in similar proportion. These results suggest that SP is an excitatory neurotransmitter in the rNTS. The effects of SP are not restricted to a particular neuron type defined either biophysically or morphologically. The implications of these results on the possible role of SP in processing gustatory and somatosensory information within the rNTS are discussed.en_US
dc.format.extent1035679 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleSubstance P excites neurons in the gustatory zone of the rat nucleus tractus solitariusen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USAen_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USAen_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USAen_US
dc.identifier.pmid7690670en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/30639/1/0000281.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-8993(93)91603-Pen_US
dc.identifier.sourceBrain Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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