Effect of mecillinam on peptidoglycan synthesis during the division cycle of Salmonella typhimurium 2616
dc.contributor.author | Licht, J. | en_US |
dc.contributor.author | Gally, D. | en_US |
dc.contributor.author | Henderson, T. | en_US |
dc.contributor.author | Young, K. | en_US |
dc.contributor.author | Cooper, Saul | en_US |
dc.date.accessioned | 2006-04-10T15:41:53Z | |
dc.date.available | 2006-04-10T15:41:53Z | |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | Licht, J., Gally, D., Henderson, T., Young, K., Cooper, S. (1993)."Effect of mecillinam on peptidoglycan synthesis during the division cycle of Salmonella typhimurium 2616." Research in Microbiology 144(6): 423-433. <http://hdl.handle.net/2027.42/30717> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6VN3-47DDBKD-9/2/8738e29efc1f57e2a1c9fa3c90c12c00 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30717 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8190989&dopt=citation | en_US |
dc.description.abstract | The effects of mecillinam, ampicillin and cephalexin on peptidoglycan synthesis in Salmonella typhimurium 2616 have been studied at equivalent concentrations or "isoactivities". Using antibiotics at isoactivities allows a direct comparison of the biochemical effects of different antibiotics. When mecillinam was added at different times during the division cycle at a concentration that produced 50 % inhibition of peptidoglycan synthesis in an exponential culture over a short period of time, the inhibition of synthesis was greatest in the newborn cells and least in the dividing cells. Antibiotic competition experiments showed that mecillinam preferentially bound to penicillin-binding protein 2 in S. typhimurium 2616. High performance liquid chromatography analysis of the residual peptidoglycan synthesized in the presence of mecillinam showed an unexpected increase in pentapeptides and a significant increase in cross-linking. Other antibiotics added at equivalent activities did not show an increase in cross-linking. | en_US |
dc.format.extent | 829191 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effect of mecillinam on peptidoglycan synthesis during the division cycle of Salmonella typhimurium 2616 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA | en_US |
dc.contributor.affiliationother | Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, ND 58202, USA | en_US |
dc.contributor.affiliationother | Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, ND 58202, USA | en_US |
dc.identifier.pmid | 8190989 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30717/1/0000363.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0923-2508(93)90050-C | en_US |
dc.identifier.source | Research in Microbiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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