Oxidation of NG-Hydroxy-L-arginine by Nitric Oxide Synthase: Evidence for the Involvement of the Heme in Catalysis
dc.contributor.author | Pufahl, R. A. | en_US |
dc.contributor.author | Marletta, M. A. | en_US |
dc.date.accessioned | 2006-05-10T15:43:27Z | |
dc.date.available | 2006-05-10T15:43:27Z | |
dc.date.issued | 1993-06-30 | en_US |
dc.identifier.citation | Pufahl R. A., , Marletta M. A., (1993/06/30)."Oxidation of NG-Hydroxy-L-arginine by Nitric Oxide Synthase: Evidence for the Involvement of the Heme in Catalysis." Biochemical and Biophysical Research Communications 193(3): 963-970. <http://hdl.handle.net/2027.42/30734> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-45PTVPB-14B/2/719a810a6fd5bc3b0841bb2a109b85c6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30734 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7686757&dopt=citation | en_US |
dc.description.abstract | The involvement of the protoporphyrin IX heme iron of macrophage nitric oxide synthase (NOS) in the oxidation of NG-hydroxy-L-arginine (L-NHA) to nitric oxide (NO) and citrulline was investigated by carbon monoxide (CO) inhibition studies and binding difference spectroscopy. A CO:oxygen mixture (80:20) was found to inhibit the reaction by 33% with L-NHA as a substrate compared to 57% with L-arginine. Spectral perturbations were observed upon the addition of L-NHA to oxidized NOS, producing a type I binding difference spectrum with a maximum at 384 nm and minimum at 420 nm. In addition, L-NHA was incapable of reducing anaerobic oxidized NOS in the absence of NADPH. These studies support the involvement of the heme in the oxidation of L-NHA to NO and citrulline, indicating that the heme functions in both of the currently characterized oxidative steps of the NOS reaction. | en_US |
dc.format.extent | 8 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.extent | 407829 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.title | Oxidation of NG-Hydroxy-L-arginine by Nitric Oxide Synthase: Evidence for the Involvement of the Heme in Catalysis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Coll Pharm, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | University of Michigan, Interdept Program Med Chem, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 7686757 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30734/3/0000383.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/bbrc.1993.1719 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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