Opioid receptor regulation of 5-hydroxytryptamine release from the rat hippocampus measured by in vivo microdialysis
dc.contributor.author | Yoshioka, Mitsuhiro | en_US |
dc.contributor.author | Matsumoto, Machiko | en_US |
dc.contributor.author | Togashi, Hiroko | en_US |
dc.contributor.author | Smith, Charles B. | en_US |
dc.contributor.author | Saito, Hideya | en_US |
dc.date.accessioned | 2006-04-10T15:43:11Z | |
dc.date.available | 2006-04-10T15:43:11Z | |
dc.date.issued | 1993-06-04 | en_US |
dc.identifier.citation | Yoshioka, Mitsuhiro, Matsumoto, Machiko, Togashi, Hiroko, Smith, Charles B., Saito, Hideya (1993/06/04)."Opioid receptor regulation of 5-hydroxytryptamine release from the rat hippocampus measured by in vivo microdialysis." Brain Research 613(1): 74-79. <http://hdl.handle.net/2027.42/30746> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-483633X-328/2/d0c502696e67261eba51092c96741d0d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30746 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8394180&dopt=citation | en_US |
dc.description.abstract | The modulation of serotonin (5-HT) release by opioid receptors in the hippocampus of the awake, unrestrained rat was evaluated by use of in vivo microdialysis. The hippocampus was perfused with Ringer's solution (2 [mu]l/min), and extracellular levels of 5-HT and its major metabolite, 5-hydroxyindoleacetic acid (5-HIAA) were estimated by assaying their concentration in the dialysate by HPLC-ECD. Addition of potassium (K+, 60 and 120 mM) to the perfusate evoked a concentration-dependent release of 5-HT, but did not alter extracellular 5-HIAA levels. Co-perfusion of morphine (0.1 to 10 [mu]M) with K+ (120 mM) produced a concentration-dependent reduction of 5-HT release. Naltrexone (0.03 to 3 mg/kg, i.p.), a relatively selective [mu]-opioid receptor antagonist, blocked in a dose-dependent manner the morphine (10 [mu]M)-induced inhibition of 5-HT release. Naltrexone alone did not alter significantly either extracellular 5-HT levels or the release of 5-HT evoked by K+. Neither co-perfusion with [-Pen2, -Pen5]-enkephalin (DPDPE, 1 to 10 [mu]M), an agonist selective for [delta]-opioid receptors, nor with U-69593 (10 [mu]M), an agonist selective for [kappa]-opioid receptors, modified the K+ (120 mM)-evoked release of 5-HT. These findings indicate that [mu]-opioid receptors modulate the physiological release of 5-HT from serotonergic neurons in the rat hippocampus. | en_US |
dc.format.extent | 620438 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Opioid receptor regulation of 5-hydroxytryptamine release from the rat hippocampus measured by in vivo microdialysis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109-0626, USA | en_US |
dc.contributor.affiliationother | First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan | en_US |
dc.contributor.affiliationother | First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan | en_US |
dc.contributor.affiliationother | First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan | en_US |
dc.contributor.affiliationother | First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan | en_US |
dc.identifier.pmid | 8394180 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30746/1/0000396.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(93)90456-W | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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