Use of Cyclodextrin-Cholesterol Complex as a Primary Standard in Cholesterol Analysis
dc.contributor.author | Karuppiah N. , | en_US |
dc.contributor.author | Kaufman P. B. , | en_US |
dc.contributor.author | Kapustka S. A. , | en_US |
dc.contributor.author | Sharma A. , | en_US |
dc.date.accessioned | 2006-04-10T15:44:21Z | |
dc.date.available | 2006-04-10T15:44:21Z | |
dc.date.issued | 1993-06 | en_US |
dc.identifier.citation | Karuppiah N., , Kaufman P. B., , Kapustka S. A., , Sharma A., (1993/06)."Use of Cyclodextrin-Cholesterol Complex as a Primary Standard in Cholesterol Analysis." Microchemical Journal 47(3): 325-329. <http://hdl.handle.net/2027.42/30771> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W6H-45P0GXS-3K/2/0b89ada073f15d156a4279fe00237d65 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30771 | |
dc.description.abstract | Cholesterol is an analyte commonly measured in various biological fluids. Enzymic procedures have virtually replaced the chemical methods for cholesterol determinations. Standardization of cholesterol assays often involve the use of solutions of this steroid containing organic solvents and/or detergents, or alternatively, the use of secondary standards. The present report shows the preparation of a primary cholesterol standard by its complexation with a chemically modified cyclodextrin. hydroxylpropyl [beta]-cyclodextrin. The use of cyclodextrin for cholesterol solubilization is not deleterious to the enzymes and other constituents present in reagents for cholesterol analysis. Such a standard is biocompatible. stable, and safe and offers an excellent alternative to the utilization of organic solvents or detergents. | en_US |
dc.format.extent | 239163 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Use of Cyclodextrin-Cholesterol Complex as a Primary Standard in Cholesterol Analysis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Department of Chemistry, Central Michigan University, Mt. Pleasant, MI 48859, USA | en_US |
dc.contributor.affiliationother | Department of Chemistry, Central Michigan University, Mt. Pleasant, MI 48859, USA | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30771/1/0000422.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/mchj.1993.1050 | en_US |
dc.identifier.source | Microchemical Journal | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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