A B2 Repeat Insertion Generates Alternate Structures of the Mouse Muscle [gamma]-Phosphorylase Kinase Gene
dc.contributor.author | Maichele, Andrea J. | en_US |
dc.contributor.author | Farwell, Nancy J. | en_US |
dc.contributor.author | Chamberlain, Jeffrey S. | en_US |
dc.date.accessioned | 2006-04-10T15:48:17Z | |
dc.date.available | 2006-04-10T15:48:17Z | |
dc.date.issued | 1993-04 | en_US |
dc.identifier.citation | Maichele, Andrea J., Farwell, Nancy J., Chamberlain, Jeffrey S. (1993/04)."A B2 Repeat Insertion Generates Alternate Structures of the Mouse Muscle [gamma]-Phosphorylase Kinase Gene." Genomics 16(1): 139-149. <http://hdl.handle.net/2027.42/30858> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-45PMT9M-Y6/2/6603718e2e81f1f39629b2a11acc84b6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30858 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8486349&dopt=citation | en_US |
dc.description.abstract | A variety of cDNA and genomic clones for the [gamma]-subunit of mouse muscle phosphorylase kinase (Phk-[gamma]M) have been isolated and characterized. The murine gene for Phk-[gamma]M (Phkg) exhibits multiple transcription start sites that are identical in skeletal muscle, cardiac muscle, and brain. The gene is composed of 10 exons and includes a 4.9-kb intron located in the 5' untranslated region. Two mRNA species of 1.75 and 2.55 kb are produced from Phkg in ICR and C57BL/10 mice; these transcripts are colinear throughout the coding region and differ only in the length of the 3' untranslated region. We have mapped the polyadenylation site of the 1.75-kb mRNA to the middle of exon 10; the 2.55-kb mRNA terminates further 3' at the end of a mouse B2 repeat. In Balb/C mice an additional B2 insertion and related genomic rearrangements alter the sequence of Phkg exon 10 and are accompanied by an increase in the quantity of the 1.75-kb transcript and a decrease in the abundance and size of the longer transcript, from 2.55 to 2.35 kb. A PCR assay for sequences contained in exon 10 reveals that the Balb/C 3' gene structure is shared by Mus musculus castaneus and Mus musculus molossinus; the C57BL/10 gene structure is shared by Mus spretus, Mus domesticus, and several strains of laboratory mice. These results suggest that Phkg in Balb/C mice was derived from M. m. molossinus and that Phkg of the other examined laboratory strains was derived from M. domesticus. | en_US |
dc.format.extent | 968098 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A B2 Repeat Insertion Generates Alternate Structures of the Mouse Muscle [gamma]-Phosphorylase Kinase Gene | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Center for Genome Technology and Genetic Disease, University of Michigan Medical School, Ann Arbor, Michigan 48109-0618 | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Center for Genome Technology and Genetic Disease, University of Michigan Medical School, Ann Arbor, Michigan 48109-0618 | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Center for Genome Technology and Genetic Disease, University of Michigan Medical School, Ann Arbor, Michigan 48109-0618 | en_US |
dc.identifier.pmid | 8486349 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30858/1/0000521.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/geno.1993.1151 | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.