pH-Dependent Decarboxylation of 2-Amino-3-ketobutyrate, the Unstable Intermediate in the Threonine Dehydrogenase-Initiated Pathway for Threonine Utilization
dc.contributor.author | Marcus, J. P. | en_US |
dc.contributor.author | Dekker, Eugene E. | en_US |
dc.date.accessioned | 2006-04-10T15:53:11Z | |
dc.date.available | 2006-04-10T15:53:11Z | |
dc.date.issued | 1993-02-15 | en_US |
dc.identifier.citation | Marcus J. P., , Dekker E. E., (1993/02/15)."pH-Dependent Decarboxylation of 2-Amino-3-ketobutyrate, the Unstable Intermediate in the Threonine Dehydrogenase-Initiated Pathway for Threonine Utilization." Biochemical and Biophysical Research Communications 190(3): 1066-1072. <http://hdl.handle.net/2027.42/30967> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-45PTW3N-6Y/2/5e4788a777fddf77d77bc514f0a3a84a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30967 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8439306&dopt=citation | en_US |
dc.description.abstract | 2-Amino-3-ketobutyrate can be readily formed enzymatically by the action of L-threonine dehydrogenase. A convenient assay for determining the half-life of this [beta]-keto acid is afforded by its rapid and quantitative conversion to glycine (+ acetyl CoA), as catalyzed by 2-amino-3-ketobutyrate CoA lyase. Using this system, we have found the half-life of 2-amino-3-ketobutyrate varies with pH from 8.6 minutes at pH 5.9 to 140 minutes at pH 11.1 yielding a theoretical titration curve that predicts a pKa value of 8.15 for the [alpha]-amino group of this intermediate. These data are considered relevant to discussions pertaining to a threonine dehydrogenase/2-amino-3-ketobutyrate CoA lyase enzyme complex in the threonine utilization pathway and to mechanistic aspects of the 5-aminolevulinate synthase-catalyzed reaction where 2-amino-3-ketoadipate is involved. | en_US |
dc.format.extent | 363982 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | pH-Dependent Decarboxylation of 2-Amino-3-ketobutyrate, the Unstable Intermediate in the Threonine Dehydrogenase-Initiated Pathway for Threonine Utilization | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 8439306 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30967/1/0000640.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/bbrc.1993.1157 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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