Progesterone modulation of gonadotropin secretion by dispersed rat pituitary cells in culture. IV. Follicle-stimulating hormone synthesis and release
dc.contributor.author | Krey, Lewis C. | en_US |
dc.contributor.author | Padmanabhan, Vasantha | en_US |
dc.contributor.author | Beitins, Inese Z. | en_US |
dc.date.accessioned | 2006-04-10T15:53:32Z | |
dc.date.available | 2006-04-10T15:53:32Z | |
dc.date.issued | 1993-02 | en_US |
dc.identifier.citation | Krey, Lewis C., Padmanabhan, Vasantha, Beitins, Inese Z. (1993/02)."Progesterone modulation of gonadotropin secretion by dispersed rat pituitary cells in culture. IV. Follicle-stimulating hormone synthesis and release." Molecular and Cellular Endocrinology 91(1-2): 13-20. <http://hdl.handle.net/2027.42/30974> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T3G-47NWGW9-KW/2/e958732a92b7dec59a4fe8147c3a87a0 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30974 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8472844&dopt=citation | en_US |
dc.description.abstract | Estradiol-treated, rat pituitary cells were studied to examine the effects of progesterone (P) on follicle-stimulating hormone (FSH) synthesis and secretion. Progesterone was administered prior to or concurrent with 3 h secretory challenges with either gonadotropin-releasing hormone (GnRH), the iontophore A23187, the protein kinase C activator phorbol 12, 13-myristate (PMA), or no secretagogue. Medium FSH levels and cell FSH stores were quantified by radioimmunoassay and bioassay. Acute (both parameters. Chronic P elevated total FSH levels even when no secretagogue was present.Studies with antiprogestins, 5[alpha]-dihydroprogesterone and 5[alpha]-reductase inhibitors revealed that this direct action of P depended on progestin receptor occupation but not on 5[alpha]-reduction. These studies indicate that P selectively increases bioactive and immunoactive FSH levels, presumably by increasing FSH synthesis, and characterize the time course and cellular mechanisms of this response. To accommodate for P modulation of total FSH levels, FSH secretion was standardized as the percentage of cellular stores available for release. Progesterone modulation of GnRH-stimulated FSH secretion was multiphasic, i.e. increased at 0-6 h, unchanged at 9 h and suppressed at 24 h. Acute and chronic exposures to P similarly modulated A23187-stimulated FSH release, whereas both P treatments increased PMA-stimulated FSH secretion. In these experiments P modulated luteinizing hormone secretion in parallel fashion, suggesting that common cellular mechanisms underlie peptidergic and steroidal regulation of the secretion of both gonadotropins. | en_US |
dc.format.extent | 860843 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Progesterone modulation of gonadotropin secretion by dispersed rat pituitary cells in culture. IV. Follicle-stimulating hormone synthesis and release | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Pediatric Endocrinology, University of Michigan Medical School, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Division of Pediatric Endocrinology, University of Michigan Medical School, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY 10021, USA | en_US |
dc.identifier.pmid | 8472844 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30974/1/0000647.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0303-7207(93)90249-J | en_US |
dc.identifier.source | Molecular and Cellular Endocrinology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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