A Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reduction
dc.contributor.author | Behbahani I. , | en_US |
dc.contributor.author | Miller S. A. , | en_US |
dc.contributor.author | Okeeffe D. H. , | en_US |
dc.date.accessioned | 2006-04-10T15:54:28Z | |
dc.date.available | 2006-04-10T15:54:28Z | |
dc.date.issued | 1993-02 | en_US |
dc.identifier.citation | Behbahani I., , Miller S. A., , Okeeffe D. H., (1993/02)."A Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reduction." Microchemical Journal 47(1-2): 251-260. <http://hdl.handle.net/2027.42/30995> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W6H-45P0GVM-37/2/2ec8e6a83bf9b37f945b5db493be7eaa | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30995 | |
dc.description.abstract | Comparisons of two assay methods for the catecholase activity of mushroom tyrosinase (EC 1.14.18.1) are reported. Tyrosinase (or polyphenol oxidase) is a widely distributed copper-containing enzyme which possesses both monooxygenase (cresolase) and oxidase (catecholase) activities. In this report the substrate employed is L-3,4-dihydroxyphenyl-alanine and the dopachrome formation and ascorbate-linked dopaquinone reduction assay methods are compared using a photodiode array spectrophotometer. This instrument has an advantage over a conventional spectrometer for kinetic studies since it is able to carry out simultaneous multiwavelength kinetic measurements at a relatively fast rate. The use of this capability in performing the two assays is described. | en_US |
dc.format.extent | 411160 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reduction | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Flint, MI, USA. | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Flint, MI, USA. | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Flint, MI, USA. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30995/1/0000670.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/mchj.1993.1040 | en_US |
dc.identifier.source | Microchemical Journal | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.