Yeast expression vectors using RNA polymerase III promoters
dc.contributor.author | Good, Paul D. | en_US |
dc.contributor.author | Engelke, David R. | en_US |
dc.date.accessioned | 2006-04-10T17:41:09Z | |
dc.date.available | 2006-04-10T17:41:09Z | |
dc.date.issued | 1994-12-30 | en_US |
dc.identifier.citation | Good, Paul D., Engelke, David R. (1994/12/30)."Yeast expression vectors using RNA polymerase III promoters." Gene 151(1-2): 209-214. <http://hdl.handle.net/2027.42/31111> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T39-47P8201-2T/2/5c44c7aa4b54643d4e08c076d3395d91 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31111 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7828876&dopt=citation | en_US |
dc.description.abstract | A series of Saccharomyces cerevisiae -- Escherichia coli shuttle vectors is described in which small RNAs can be stably expressed in yeast from two different promoters for RNA polymerase III transcription. The vectors are available in either high- or low-copy-number forms with either URA3, HIS3, or TRP1 selection markers, and are based on a previously described set of plasmid vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27]. Transcripts have structured pre-tRNA or RPR1 leaders fused to RNA corresponding to inserted sequences. Levels of RNA accumulation are dependent on plasmid copy number and the type of transcript. | en_US |
dc.format.extent | 676964 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Yeast expression vectors using RNA polymerase III promoters | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-0606, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-0606, USA | en_US |
dc.identifier.pmid | 7828876 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31111/1/0000007.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1119(94)90658-0 | en_US |
dc.identifier.source | Gene | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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