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Yeast expression vectors using RNA polymerase III promoters

dc.contributor.authorGood, Paul D.en_US
dc.contributor.authorEngelke, David R.en_US
dc.date.accessioned2006-04-10T17:41:09Z
dc.date.available2006-04-10T17:41:09Z
dc.date.issued1994-12-30en_US
dc.identifier.citationGood, Paul D., Engelke, David R. (1994/12/30)."Yeast expression vectors using RNA polymerase III promoters." Gene 151(1-2): 209-214. <http://hdl.handle.net/2027.42/31111>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T39-47P8201-2T/2/5c44c7aa4b54643d4e08c076d3395d91en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/31111
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7828876&dopt=citationen_US
dc.description.abstractA series of Saccharomyces cerevisiae -- Escherichia coli shuttle vectors is described in which small RNAs can be stably expressed in yeast from two different promoters for RNA polymerase III transcription. The vectors are available in either high- or low-copy-number forms with either URA3, HIS3, or TRP1 selection markers, and are based on a previously described set of plasmid vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27]. Transcripts have structured pre-tRNA or RPR1 leaders fused to RNA corresponding to inserted sequences. Levels of RNA accumulation are dependent on plasmid copy number and the type of transcript.en_US
dc.format.extent676964 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleYeast expression vectors using RNA polymerase III promotersen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-0606, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-0606, USAen_US
dc.identifier.pmid7828876en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/31111/1/0000007.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0378-1119(94)90658-0en_US
dc.identifier.sourceGeneen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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