View Item 

Show simple item record

Rapid kinetics of G protein subunit association: A rate-limiting conformational change?
dc.contributor.authorNeubig, Richard R.en_US
dc.contributor.authorConnolly, Mark P.en_US
dc.contributor.authorRemmers, Ann E.en_US
dc.date.accessioned2006-04-10T17:42:29Z
dc.date.available2006-04-10T17:42:29Z
dc.date.issued1994-12-05en_US
dc.identifier.citationNeubig, Richard R., Connolly, Mark P., Remmers, Ann E. (1994/12/05)."Rapid kinetics of G protein subunit association: A rate-limiting conformational change?." FEBS Letters 355(3): 251-253. <http://hdl.handle.net/2027.42/31136>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T36-447MXGH-8K/2/b49e0b989714887367af2f14eca3f61fen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/31136
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7527348&dopt=citationen_US
dc.description.abstractG protein subunit association and dissociation are thought to play an important role in signal transduction. We measured [alpha][beta][gamma] heterocomplex formation using resonance energy transfer. Fluorescein-labelled [alpha](F-[alpha]) emission was quenched ~ 10% on mixing with eosin-labelled [beta][gamma](E-[beta][gamma]). Unlabelled [beta][gamma] did not quench F-[alpha] fluorescence. Stopped-flow kinetics showed a t1/2 ranging from 2.5 s to 0.25 s for 50 nM to 1200 nM E-[beta][gamma]. The rate saturated at high E-[beta][gamma] concentrations consistent with a two-step mechanism. We report the first rapid-mix studies of G protein subunit association kinetics which suggest that [alpha] and [beta][gamma] combine by a two-step process with a maximal rate of 4.1 +/- 0.4 s-1.en_US
dc.format.extent346367 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleRapid kinetics of G protein subunit association: A rate-limiting conformational change?en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan, 1301 M.S.R.B. III, Ann Arbor, MI 48109-0632, USAen_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan, 1301 M.S.R.B. III, Ann Arbor, MI 48109-0632, USAen_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan, 1301 M.S.R.B. III, Ann Arbor, MI 48109-0632, USAen_US
dc.identifier.pmid7527348en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/31136/1/0000033.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0014-5793(94)01212-1en_US
dc.identifier.sourceFEBS Lettersen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000033.pdf
Size:
338.2KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.