View Item 

Show simple item record

Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays

dc.contributor.authorBuckwalter, Jeanette M.en_US
dc.contributor.authorGuo, Xuanen_US
dc.contributor.authorMeyerhoff, Mark E.en_US
dc.date.accessioned2006-04-10T17:45:11Z
dc.date.available2006-04-10T17:45:11Z
dc.date.issued1994-11-20en_US
dc.identifier.citationBuckwalter, Jeanette M., Guo, Xuan, Meyerhoff, Mark E. (1994/11/20)."Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays." Analytica Chimica Acta 298(1): 11-18. <http://hdl.handle.net/2027.42/31186>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6TF4-4BJNRPK-M/2/ec47f75ac7a397a4b1225f7c22bada3een_US
dc.identifier.urihttps://hdl.handle.net/2027.42/31186
dc.description.abstractThe use of two enzyme labels in a dual analyte simultaneous heterogeneous enzyme-linked competitive binding assay is examined. Reaction conditions for monitoring glucose-6-phosphate dehydrogenase (G6PDH) and [beta]-galactosidase ([beta]-gal) activities are found which enable the independent measurement of each enzyme activity in the presence of the other. The two enzymes are used as labels in the development of a model simultaneous heterogeneous competitive binding assay for detecting the biotin and vitamin B12 content of vitamin tablets. The simultaneous assay is shown to exhibit analytical dose-response characteristics comparable to those of the single analyte assays. The potential use of the G6PDH/[beta]-gal enzyme pair for devising other dual analyte competitive and non-competitive binding assays is discussed.en_US
dc.format.extent745584 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleDual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assaysen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USAen_US
dc.contributor.affiliationumDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USAen_US
dc.contributor.affiliationumDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USAen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/31186/1/0000087.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2670(94)90038-8en_US
dc.identifier.sourceAnalytica Chimica Actaen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000087.pdf
Size:
728.1KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.