Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays
dc.contributor.author | Buckwalter, Jeanette M. | en_US |
dc.contributor.author | Guo, Xuan | en_US |
dc.contributor.author | Meyerhoff, Mark E. | en_US |
dc.date.accessioned | 2006-04-10T17:45:11Z | |
dc.date.available | 2006-04-10T17:45:11Z | |
dc.date.issued | 1994-11-20 | en_US |
dc.identifier.citation | Buckwalter, Jeanette M., Guo, Xuan, Meyerhoff, Mark E. (1994/11/20)."Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays." Analytica Chimica Acta 298(1): 11-18. <http://hdl.handle.net/2027.42/31186> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TF4-4BJNRPK-M/2/ec47f75ac7a397a4b1225f7c22bada3e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31186 | |
dc.description.abstract | The use of two enzyme labels in a dual analyte simultaneous heterogeneous enzyme-linked competitive binding assay is examined. Reaction conditions for monitoring glucose-6-phosphate dehydrogenase (G6PDH) and [beta]-galactosidase ([beta]-gal) activities are found which enable the independent measurement of each enzyme activity in the presence of the other. The two enzymes are used as labels in the development of a model simultaneous heterogeneous competitive binding assay for detecting the biotin and vitamin B12 content of vitamin tablets. The simultaneous assay is shown to exhibit analytical dose-response characteristics comparable to those of the single analyte assays. The potential use of the G6PDH/[beta]-gal enzyme pair for devising other dual analyte competitive and non-competitive binding assays is discussed. | en_US |
dc.format.extent | 745584 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31186/1/0000087.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2670(94)90038-8 | en_US |
dc.identifier.source | Analytica Chimica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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