[alpha]-(2 --> 3)- and [alpha]-(2 --> 6)-Sialyltransferase activities present in three variants of Ehrlich tumor cells: identification of the products derived from N-acetyllactosamine and [beta]--Gal-(1 --> 3)-[alpha] GalNAc-(1 --> O)-Bn
dc.contributor.author | Shigeta, Seiko | en_US |
dc.contributor.author | Winter, Harry C. | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-10T17:46:10Z | |
dc.date.available | 2006-04-10T17:46:10Z | |
dc.date.issued | 1994-11-01 | en_US |
dc.identifier.citation | Shigeta, Seiko, Winter, Harry C., Goldstein, Irwin J. (1994/11/01)."[alpha]-(2 --> 3)- and [alpha]-(2 --> 6)-Sialyltransferase activities present in three variants of Ehrlich tumor cells: identification of the products derived from N-acetyllactosamine and [beta]--Gal-(1 --> 3)-[alpha] GalNAc-(1 --> O)-Bn." Carbohydrate Research 264(1): 111-121. <http://hdl.handle.net/2027.42/31201> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TFF-43T8RCF-X/2/b61d115dbf87b0aa82fb3ae58f953262 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31201 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8001013&dopt=citation | en_US |
dc.description.abstract | We compared several sialyltransferase activities related to synthesis of O-linked and N-linked sialylglycoproteins in Ehrlich ascites tumor cells that grow normally in murine ascites, but are not adherent nor grow in tissue culture (na-EAT cells), with those in cells that were selected to grow in tissue culture and adhere to extracellular matrices (a-EAT cells). Crude Golgi preparations from both cell types contained predominantly [beta]--Gal-(1 --> 3)--GalNAc [alpha]-(2 --> 3)-sialyltransferase activity. Sialylation of N-acetyllactosamine, lacto-N-tetraose, and benzyl [alpha]--Ga1NAc occurred at from 1 to 4% of that activity. Analysis, by ion-exchange HPLC at high pH, of sialylated N-acetyllactosamine showed that na-EAT cells sialylated [beta]--Gal-(1 --> 4)--GlcNAc mostly by [alpha]-(2 --> 3)-sialyltransferase, whereas [beta]--Gal-(1 --> 4)--GlcNAc [alpha]-(2 --> 6)-sialyltransferase activity was prominent in a-EAT cells. In addition, preparations from na-EAT cells formed significant quantities of an unknown tritiated product from CMP-[9-3H]sialic acid, suggesting at least one other difference in enzyme levels between the cell types. a-EAT cells reestablished in murine ascites for 11 passages retained the sialyltransferase levels characteristic of a-EAT cells. When viable cells were labeled with | en_US |
dc.format.extent | 964285 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | [alpha]-(2 --> 3)- and [alpha]-(2 --> 6)-Sialyltransferase activities present in three variants of Ehrlich tumor cells: identification of the products derived from N-acetyllactosamine and [beta]--Gal-(1 --> 3)-[alpha] GalNAc-(1 --> O)-Bn | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biochemistry, University of Michigan Medical School 1301 Catherine Road, Ann Arbor, MI 48109-0606, USA | en_US |
dc.contributor.affiliationum | Department of Biochemistry, University of Michigan Medical School 1301 Catherine Road, Ann Arbor, MI 48109-0606, USA | en_US |
dc.contributor.affiliationum | Department of Biochemistry, University of Michigan Medical School 1301 Catherine Road, Ann Arbor, MI 48109-0606, USA | en_US |
dc.identifier.pmid | 8001013 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31201/1/0000103.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0008-6215(94)00192-8 | en_US |
dc.identifier.source | Carbohydrate Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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