Lack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp-->Cys or Trp-->Phemutants of human carbonic anhydrase II
dc.contributor.author | Bergenhem, Nils C. H. | en_US |
dc.contributor.author | Schlyer, Bruce D. | en_US |
dc.contributor.author | Steel, Duncan G. | en_US |
dc.contributor.author | Gafni, Ari | en_US |
dc.contributor.author | Carlsson, Uno | en_US |
dc.contributor.author | Jonsson, Bengt-Harald | en_US |
dc.date.accessioned | 2006-04-10T17:49:21Z | |
dc.date.available | 2006-04-10T17:49:21Z | |
dc.date.issued | 1994-10-17 | en_US |
dc.identifier.citation | Bergenhem, Nils C. H., Schlyer, Bruce D., Steel, Duncan G., Gafni, Ari, Carlsson, Uno, Jonsson, Bengt-Harald (1994/10/17)."Lack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp-->Cys or Trp-->Phemutants of human carbonic anhydrase II." FEBS Letters 353(2): 177-179. <http://hdl.handle.net/2027.42/31251> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T36-447MX41-4X/2/00d07af8c24f10c0096dc37c7f6ff813 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31251 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7926047&dopt=citation | en_US |
dc.description.abstract | The room temperature phosphorescence of native human carbonic anhydrase (CA), and several mutants of this enzyme has been investigated. In these mutants the seven tryptophan residues in the native protein have sequentially been replaced by cysteine or phenylalanine. All of the mutants as well as native CA show room-temperature tryptophan phosphorescence (RTP) spectra. Surprisingly, only small differences in RTP life-times are noticeable among these mutants, indicating that there is more than one tryptophan residue with similar phosphorescence decay kinetics in the protein. The present results illustrate the danger in attributing the room temperature phosphorescence of a multi-tryptophan protein to a particular residue based solely on an analysis of the protein structure. | en_US |
dc.format.extent | 280183 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Lack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp-->Cys or Trp-->Phemutants of human carbonic anhydrase II | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA | en_US |
dc.contributor.affiliationum | Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA; Department of Biological Chemistry University of Michigan, Ann Arbor, MI 48109-2007, USA. | en_US |
dc.contributor.affiliationum | Department of Physics and Electrical Engineering, University of Michigan, Ann Arbor, MI 48109-2007, USA; Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry University of Michigan, Ann Arbor, MI 48109-2007, USA; Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA. | en_US |
dc.contributor.affiliationother | IFM/Department of Chemistry, Linköping University, S-581 83 Linköping, Sweden | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Umeå University, S-901 87, Umeå, Sweden | en_US |
dc.identifier.pmid | 7926047 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31251/1/0000157.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-5793(94)01041-2 | en_US |
dc.identifier.source | FEBS Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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