High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone
dc.contributor.author | van den Berg, Joop D. | en_US |
dc.contributor.author | Smets, Lou A. | en_US |
dc.contributor.author | Hutchison, Kevin A. | en_US |
dc.contributor.author | van Rooij, Henny | en_US |
dc.contributor.author | van den Elshout, Marion M. | en_US |
dc.date.accessioned | 2006-04-10T17:50:32Z | |
dc.date.available | 2006-04-10T17:50:32Z | |
dc.date.issued | 1994-10 | en_US |
dc.identifier.citation | van den Berg, Joop D., Smets, Lou A., Hutchison, Kevin A., van Rooij, Henny, van den Elshout, Marion M. (1994/10)."High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone." The Journal of Steroid Biochemistry and Molecular Biology 51(1-2): 33-40. <http://hdl.handle.net/2027.42/31269> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T8X-47PR3KV-N2/2/95da8773fbffbf10003ddb80b1697930 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31269 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7947348&dopt=citation | en_US |
dc.description.abstract | Upon agonist binding the heteromeric glucocorticoid receptor complex under goes a conformational change (receptor activation). This event involves the dissociation of a dimer of 90 kDa heat shock proteins. Whereas receptor activation in cytosolic assays is both rapid and irreversible, less is known about the receptor activation and translocation in intact cells during challenge with an agonist. In this paper we report on the receptor status of glucocorticoid-sensitive murine S49 lymphoma cells during dexamethasone exposure. By three different assays, ligand (re)binding, nuclear translocation and hsp90 co-immunoprecipitation, it was found that the majority of the glucocorticoid receptor protein was in a non-activated conformation. Furthermore, prolonged exposure to dexamethasone did not result in increased levels of activated receptors. By assessing receptor activation in situ we found that physiological temperature was less effective in dissociating hsp90 compared to room temperature. These findings indicate that the physiological temperature negatively controls receptor activation, probably due to a thermolabile interaction between the hormone and its cognate receptor. | en_US |
dc.format.extent | 984040 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Medical Science Building I, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | The Netherlands Cancer Institute/Antoni van Leeuwenhoekhuis, Division of Experimental Therapy, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands | en_US |
dc.contributor.affiliationother | The Netherlands Cancer Institute/Antoni van Leeuwenhoekhuis, Division of Experimental Therapy, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands | en_US |
dc.contributor.affiliationother | The Netherlands Cancer Institute/Antoni van Leeuwenhoekhuis, Division of Experimental Therapy, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands | en_US |
dc.contributor.affiliationother | The Netherlands Cancer Institute/Antoni van Leeuwenhoekhuis, Division of Experimental Therapy, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands | en_US |
dc.identifier.pmid | 7947348 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31269/1/0000175.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0960-0760(94)90112-0 | en_US |
dc.identifier.source | The Journal of Steroid Biochemistry and Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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