Keratinocyte Interleukin-10 Expression Is Upregulated in Tape-Stripped Skin, Poison Ivy Dermatitis, and Sezary Syndrome, but Not in Psoriatic Plaques
dc.contributor.author | Nickoloff, Brian J. | en_US |
dc.contributor.author | Fivenson, David P. | en_US |
dc.contributor.author | Kunkel, Steven L. | en_US |
dc.contributor.author | Strieter, Robert M. | en_US |
dc.contributor.author | Turka, Laurence A. | en_US |
dc.date.accessioned | 2006-04-10T17:50:46Z | |
dc.date.available | 2006-04-10T17:50:46Z | |
dc.date.issued | 1994-10 | en_US |
dc.identifier.citation | Nickoloff, Brian J., Fivenson, David P., Kunkel, Steven L., Strieter, Robert M., Turka, Laurence A. (1994/10)."Keratinocyte Interleukin-10 Expression Is Upregulated in Tape-Stripped Skin, Poison Ivy Dermatitis, and Sezary Syndrome, but Not in Psoriatic Plaques." Clinical Immunology and Immunopathology 73(1): 63-68. <http://hdl.handle.net/2027.42/31273> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCK-45P0WGW-1C/2/1f2d5dcf5caf14dc94b51784e6b2f8e8 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31273 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7923918&dopt=citation | en_US |
dc.description.abstract | Despite the highly diverse reaction patterns of benign and malignant skin diseases involving T lymphocytes, polymerase chain reaction analysis of cytokine mRNAs present in biopsy samples has revealed that many cutaneous responses can be categorized into essentially two discrete groups. One group exemplified by psoriasis is characterized by consistently detectable mRNAs for IL-2, IFN-[gamma], and TNF-[alpha], but not IL-4, IL-5, IL-10, thereby closely resembling the murine Th1-type cell-mediated response. The second group exemplified by tape-stripped skin, poison ivy dermatitis, and Sezary syndrome contains predominantly IL-4, IL-5, and IL-10 mRNAs resembling the Th2-type cytokine profile. Because of the growing interest in the immunoregulatory role of IL-10, which can suppress IFN-[gamma] production and inhibit cell-mediated reactions, we produced a rabbit antiserum that was used to immunohistochemically localize IL-10 in a total of 27 biopsies. The results revealed that in Th2-type skin diseases, IL-10 was predominantly identified throughout all levels of epidermis in the cytoplasm of keratinocytes (KCs), with accentuation of their membranes in upper level cells. In Sezary syndrome, T cells were also immunoreactive for IL-10, which was confirmed using the HUT 78 T cell line derived from a Sezary syndrome patient. While normal skin was devoid of IL-10 expression, KCs began expressing it as early as 6 hr following tape stripping or application of poison ivy antigen and became strongly and diffusely positive by 18-24 hr. In contrast, psoriatic plaques contained no IL-10 immunoreactivity in either the parakeratotic scale or the epidermal KCs. These results confirm the earlier IL-10 mRNA analysis using whole skin samples and immunolocalize IL-10 to epidermal KCs in the Th2 diseases. | en_US |
dc.format.extent | 538535 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Keratinocyte Interleukin-10 Expression Is Upregulated in Tape-Stripped Skin, Poison Ivy Dermatitis, and Sezary Syndrome, but Not in Psoriatic Plaques | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationother | Department of Dermatology, Henry Ford Hospital, Detroit, Michigan, USA. | en_US |
dc.identifier.pmid | 7923918 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31273/1/0000179.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/clin.1994.1170 | en_US |
dc.identifier.source | Clinical Immunology and Immunopathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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