Cloning and characterization of a pharmacologically distinct A1 adenosine receptor from guinea pig brain
dc.contributor.author | Xie, Fan Meng Guo-xi | en_US |
dc.contributor.author | Chalmers, Derek T. | en_US |
dc.contributor.author | Morgan, Caurnel | en_US |
dc.contributor.author | Watson, Stanley J. | en_US |
dc.contributor.author | Akil, Huda | en_US |
dc.date.accessioned | 2006-04-10T17:51:17Z | |
dc.date.available | 2006-04-10T17:51:17Z | |
dc.date.issued | 1994-10 | en_US |
dc.identifier.citation | Xie, Fan Meng Guo-xi, Chalmers, Derek, Morgan, Caurnel, Watson, Jr., Stanley J., Akil, Huda (1994/10)."Cloning and characterization of a pharmacologically distinct A1 adenosine receptor from guinea pig brain." Molecular Brain Research 26(1-2): 143-155. <http://hdl.handle.net/2027.42/31281> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T07-485RK1F-P/2/c7cf7629d623dcd94a403ac91efc0303 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31281 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7854041&dopt=citation | en_US |
dc.description.abstract | Three full-length cDNA clones encoding the guinea pig A1 adenosine receptor have been isolated by polymerase chain reaction (PCR) and low-stringency hybridization screening of a guinea pig brain cDNA library. These three cDNAs, though differing in their 5' untranslated regions, contain the same open reading frame encoding a 326 amino acid residue protein with seven hydrophobic [alpha]-helices long enough to form the transmembrane domains, suggesting that it belongs to the G protein-coupled receptor superfamily. This protein is highly homologous to the A1 adenosine receptors previously cloned from other species. Pharmacological characterization of this receptor transiently expressed in mammalian cells demonstrates that, despite its high homology to A1 adenosine receptors of other species, the guinea pig A1 adenosine receptor displays a unique pharmacological profile: high affinity for the A1-selective antagonist CPX, yet very low affinity for some A1-selective agonists such as CCPA, CHA and R-PIA. Northern blotting for different guinea pig tissues and in situ hybridization for guinea pig brain sections reveal an abundant and broad distribution of mRNA of this A1 subtype receptor in the brain. | en_US |
dc.format.extent | 1290762 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cloning and characterization of a pharmacologically distinct A1 adenosine receptor from guinea pig brain | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 7854041 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31281/1/0000187.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0169-328X(94)90085-X | en_US |
dc.identifier.source | Molecular Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.