Effects of antimalarials and protease inhibitors on plasmodial hemozoin production
dc.contributor.author | Asawamabasakda, Wanida | en_US |
dc.contributor.author | Ittarat, Isra | en_US |
dc.contributor.author | Chang, Chih-Ching | en_US |
dc.contributor.author | McElroy, Peter | en_US |
dc.contributor.author | Meshnick, Steven R. | en_US |
dc.date.accessioned | 2006-04-10T17:51:30Z | |
dc.date.available | 2006-04-10T17:51:30Z | |
dc.date.issued | 1994-10 | en_US |
dc.identifier.citation | Asawamabasakda, Wanida, Ittarat, Isra, Chang, Chih-Ching, McElroy, Peter, Meshnick, Steven R. (1994/10)."Effects of antimalarials and protease inhibitors on plasmodial hemozoin production." Molecular and Biochemical Parasitology 67(2): 183-191. <http://hdl.handle.net/2027.42/31285> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T29-476TY70-P/2/4657d0376b86a4495e65c6c98dbf877e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31285 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7870123&dopt=citation | en_US |
dc.description.abstract | Malarial hemozoin may play an important role as a target for antimalarial drugs and in disease pathogenesis. A new assay for hemozoin was developed in which the hemozoin was separated from cells by filtration. Trophozoites have substantially more hemozoin than rings, but there are relatively small differences between chloroquine-sensitive and chloroquine-resistant strains. The effects on hemozoin content of chloroquine and artemisinin, two antimalarial drugs, and E64 and Pepstatin A, two protease inhibitors, were measured. At concentrations at which hypoxanthine incorporation was unaffected, the hemozoin content of rings was decreased by E64, but not by the other three compounds. Artemisinin and Pepstatin A also had little effect on the hemozoin content of trophozoites. Chloroquine and E64 inhibited trophozoite hemozoin formation, but inhibited hypoxanthine uptake to a similar or greater extent. When either rings or trophozoites were exposed to several higher concentrations of chloroquine, hemozoin content was diminished, but significantly less than hypoxanthine uptake. Various concentrations of E64, in contrast, inhibited hemozoin production by both rings and trophozoites significantly more than hypoxanthine incorporation, suggesting that hemozoin production may be directly affected by E64. | en_US |
dc.format.extent | 680441 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effects of antimalarials and protease inhibitors on plasmodial hemozoin production | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Epidemiology, University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 7870123 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31285/1/0000191.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0166-6851(94)00128-6 | en_US |
dc.identifier.source | Molecular and Biochemical Parasitology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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