The NH2-Terminal Region of Rabbit CYP2E1 Is Not Essential for Interaction with NADPH-Cytochrome P450 Reductase
dc.contributor.author | Voznesensky A. I. , | en_US |
dc.contributor.author | Schenkman J. B. , | en_US |
dc.contributor.author | Pernecky S. J. , | en_US |
dc.contributor.author | Coon M. J. , | en_US |
dc.date.accessioned | 2006-05-10T15:34:20Z | |
dc.date.available | 2006-05-10T15:34:20Z | |
dc.date.issued | 1994-08-30 | en_US |
dc.identifier.citation | Voznesensky A. I., , Schenkman J. B., , Pernecky S. J., , Coon M. J., (1994/08/30)."The NH2-Terminal Region of Rabbit CYP2E1 Is Not Essential for Interaction with NADPH-Cytochrome P450 Reductase." Biochemical and Biophysical Research Communications 203(1): 156-161. <http://hdl.handle.net/2027.42/31380> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-45PMM58-J2/2/c2d6ce6d74a76584fc56920096595040 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31380 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8074649&dopt=citation | en_US |
dc.description.abstract | Recently we reported that electrostatic forces interfere with the formation of the NADPH-cytochrome P450 reductase/cytochrome P450 electron transfer complex and suggested that this complex is formed by the attraction of the complementary hydrophobic patches (Voznesensky, A., and Schenkman,J.(1994) 269 J.Biol.Chem, 15724-1573). In this report we evaluate the role of the NH2-terminal hydrophobic region of CYP2E1 in the interaction with the reductase by comparing ionic strength dependence of the reduction of the full-length and truncated [Delta]3-29 CYP2E1. Increasing ionic strength stimulates reduction of both full-length and truncated CYP2E1. The neutralization of electrostatic interactions by increasing ionic strength revealed no impairment of the [Delta]3-29 CYP2E1 reduction compared to that of the full-length CYP2E1, indicating that the NH2-terminal region is not essential for the interaction of the cytochrome with the reductase. | en_US |
dc.format.extent | 6 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.extent | 304069 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.title | The NH2-Terminal Region of Rabbit CYP2E1 Is Not Essential for Interaction with NADPH-Cytochrome P450 Reductase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030, USA and University of Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030, USA and University of Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030, USA and University of Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030, USA and University of Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 8074649 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31380/3/0000293.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/bbrc.1994.2162 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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