Enhanced diagnostic efficiency of the polymerase chain reaction by co-amplification of multiple regions of HIV-1 and HIV-2
dc.contributor.author | Udaykumar, | en_US |
dc.contributor.author | Heredia, Alonso | en_US |
dc.contributor.author | Soriano, Vincent | en_US |
dc.contributor.author | Bravo, Rosa | en_US |
dc.contributor.author | Epstein, Jay S. | en_US |
dc.contributor.author | Hewlett, Indira K. | en_US |
dc.date.accessioned | 2006-04-10T17:57:52Z | |
dc.date.available | 2006-04-10T17:57:52Z | |
dc.date.issued | 1994-08 | en_US |
dc.identifier.citation | Udaykumar, , Heredia, Alonso, Soriano, Vincent, Bravo, Rosa, Epstein, Jay S., Hewlett, Indira K. (1994/08)."Enhanced diagnostic efficiency of the polymerase chain reaction by co-amplification of multiple regions of HIV-1 and HIV-2." Journal of Virological Methods 49(1): 37-46. <http://hdl.handle.net/2027.42/31395> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T96-476F6X3-KP/2/3b2bae6bb7f4f3abc073f7b17eda650b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31395 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7829590&dopt=citation | en_US |
dc.description.abstract | A method for co-amplification of multiple viral sequences of HIV-1 and HIV-2 by polymerase chain reaction was designed. The technique resulted in the specific detection of each type of virus and allowed the amplification of as few as two copies of target DNA. The amplification of multiple regions of the viral genome offers the advantage of detecting multiple target sequences, which may be essential for some viruses, such as HIV, that exhibit a high degree of variability in their gene sequences. In addition, the method permitted the detection of both virus types in the same reaction, allowing for greater utility in geographic areas where coinfections with both viruses occur and cross-reactivity in Western blots is observed. This method was applied successfully to the detection of viral DNA in clinical specimens. | en_US |
dc.format.extent | 885390 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Enhanced diagnostic efficiency of the polymerase chain reaction by co-amplification of multiple regions of HIV-1 and HIV-2 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The University of Michigan Medical Center, MSRB 1, Room 4510, 1150 West Medical Center Drive, Ann Arbor, Ml 48109-0650, USA | en_US |
dc.contributor.affiliationother | Laboratory of Molecular Virology, Division of Transfusion and Transmitted Diseases, Center for Biologies Evaluation and Research, Food and Drug Administration, 1401 Roekville Pike, Rockville, MD 20852-1448, USA | en_US |
dc.contributor.affiliationother | Service of Infectious Diseases, Microbiology and Immunology, Instituto de Salud Carlos III, 28010, Madrid, Spain | en_US |
dc.contributor.affiliationother | Service of Infectious Diseases, Microbiology and Immunology, Instituto de Salud Carlos III, 28010, Madrid, Spain | en_US |
dc.contributor.affiliationother | Laboratory of Molecular Virology, Division of Transfusion and Transmitted Diseases, Center for Biologies Evaluation and Research, Food and Drug Administration, 1401 Roekville Pike, Rockville, MD 20852-1448, USA | en_US |
dc.contributor.affiliationother | Laboratory of Molecular Virology, Division of Transfusion and Transmitted Diseases, Center for Biologies Evaluation and Research, Food and Drug Administration, 1401 Roekville Pike, Rockville, MD 20852-1448, USA | en_US |
dc.identifier.pmid | 7829590 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31395/1/0000309.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0166-0934(94)90053-1 | en_US |
dc.identifier.source | Journal of Virological Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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