Multicolor FISH Mapping of YAC Clones in 3p14 and Identification of a YAC Spanning both FRA3B and the t(3;8) Associated with Hereditary Renal Cell Carcinoma
dc.contributor.author | Wilke, Charles M. | en_US |
dc.contributor.author | Guo, Sun-Wei | en_US |
dc.contributor.author | Hall, Bryan K. | en_US |
dc.contributor.author | Boldog, Ferenc | en_US |
dc.contributor.author | Gemmill, Robert M. | en_US |
dc.contributor.author | Chandrasekharappa, Settara C. | en_US |
dc.contributor.author | Barcroft, Christine L. | en_US |
dc.contributor.author | Drabkin, Harry A. | en_US |
dc.contributor.author | Glover, Thomas W. | en_US |
dc.date.accessioned | 2006-04-10T18:00:25Z | |
dc.date.available | 2006-04-10T18:00:25Z | |
dc.date.issued | 1994-07-15 | en_US |
dc.identifier.citation | Wilke, Charles M., Guo, Sun-Wei, Hall, Bryan K., Boldog, Ferenc, Gemmill, Robert M., Chandrasekharappa, Settara C., Barcroft, Christine L., Drabkin, Harry A., Glover, Thomas W. (1994/07/15)."Multicolor FISH Mapping of YAC Clones in 3p14 and Identification of a YAC Spanning both FRA3B and the t(3;8) Associated with Hereditary Renal Cell Carcinoma." Genomics 22(2): 319-326. <http://hdl.handle.net/2027.42/31440> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-45NJXHJ-1C/2/658f5b18ce3204c13729ce93b13b1059 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31440 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7806217&dopt=citation | en_US |
dc.description.abstract | Human chromosome band 3p14 contains two tightly linked cytogenetic markers of broad interest, FRA3B and the t(3;8) breakpoint associated with hereditary renal cell carcinoma (RCC). The common fragile site at 3p14.2 (FRA3B) is the most sensitive site on normal human chromosomes to breakage when DNA replication is perturbed by aphidicolin or folate stress. The t(3;8) (p14.2;q24.1) translocation segregates with RCC in a large family and could mark the location of a tumor suppressor gene involved in renal cancers. In studies aimed at positional cloning of FRA3B and the t(3;8) breakpoint, we have used multicolor fluorescence in situ hybridization analysis (FISH) on metaphase spreads and interphase nuclei to order 14 yeast artificial chromosomes (YACs) in 3p14. The YACs used in this study were identified by a group of unordered lambda clones that had been previously localized to the 3p14 region and mapped proximal or distal to the t(3;8) breakpoint. FISH analysis was used to order the YACs and to map them in relation both to the t(3;8) translocation breakpoint and to FRA3B induced on normal chromosomes by treatment with aphidicolin. YACs that closely flanked both the t(3;8) translocation breakpoint and the fragile site were identified. A YAC walk from the closest distal YAC allowed the identification of a 1.3-Mb YAC derived from the CEPH large insert YAC library that spans both the FRA3B and the t(3;8) breakpoint. The order of the YACs and cytogenetic landmarks in 3p14 is cen-(126E1/230B9)-181H6-B15-D20F4-258B7-280D2-70E12-168A8-403B2-143C5-413C6-468B10-[850A6/t(3;8)/FRA3B]-74B2. The location of FRA3B and the t(3;8) translocation within the same YAC supports previous cytogenetic studies indicating that these two sites are very closely linked and provides a resource for their molecular analyses. | en_US |
dc.format.extent | 564889 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Multicolor FISH Mapping of YAC Clones in 3p14 and Identification of a YAC Spanning both FRA3B and the t(3;8) Associated with Hereditary Renal Cell Carcinoma | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Department of Pediatrics | en_US |
dc.contributor.affiliationum | University of Michigan Department Department of Biostatistics | en_US |
dc.contributor.affiliationum | University of Michigan Department of Human Genetics | en_US |
dc.contributor.affiliationum | University of Michigan Human Genome Center, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | University of Michigan Department of Pediatrics | en_US |
dc.contributor.affiliationother | University of Colorado Cancer Center, Division of Medical Oncology, and Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado | en_US |
dc.contributor.affiliationother | University of Colorado Cancer Center, Division of Medical Oncology, and Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado | en_US |
dc.contributor.affiliationother | Department of Pediatrics and Human Genome Center, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | University of Colorado Cancer Center, Division of Medical Oncology | en_US |
dc.identifier.pmid | 7806217 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31440/1/0000358.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/geno.1994.1390 | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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