Regulation of Chemokine Production by the Oxidative Metabolism of L-Arginine in a Human Mixed Lymphocyte Reaction
dc.contributor.author | Orens, Jonathan B. | en_US |
dc.contributor.author | Lukacs, Nicholas W. | en_US |
dc.contributor.author | Kunkel, Steven L. | en_US |
dc.contributor.author | Burdick, Marie D. | en_US |
dc.contributor.author | Wilke, Carol A. | en_US |
dc.contributor.author | Walz, Alfred | en_US |
dc.contributor.author | Strieter, Robert M. | en_US |
dc.date.accessioned | 2006-04-10T18:05:04Z | |
dc.date.available | 2006-04-10T18:05:04Z | |
dc.date.issued | 1994-06 | en_US |
dc.identifier.citation | Orens, Jonathan B., Lukacs, Nicholas W., Kunkel, Steven L., Burdick, Marie D., Wilke, Carol A., Walz, Alfred, Strieter, Robert M. (1994/06)."Regulation of Chemokine Production by the Oxidative Metabolism of L-Arginine in a Human Mixed Lymphocyte Reaction." Cellular Immunology 156(1): 95-101. <http://hdl.handle.net/2027.42/31513> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCF-45P0VS0-5M/2/e4292f0b39cfc295bd549743e866f06d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31513 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8200045&dopt=citation | en_US |
dc.description.abstract | Previous studies have shown that during the development of a mixed lymphocyte reaction (MLR) levels of the chemotactic cytokines IL-8 and MCP-1 (members of the C-X-C and C-C supergene families, respectively) increase in a time-dependent fashion, and that the production of these chemokines correlates with the magnitude of responsiveness to alloantigen (13). Furthermore, the responsiveness to alloantigen in the context of a MLR has been shown to be regulated by the oxidative metabolism of L-arginine (12). We postulated that competitive antagonism of the L-arginine metabolic pathway in a human MLR may alter the production of members of the C-C and C-X-C chemokine families. To test this hypothesis, mononuclear cells were isolated from healthy individuals and subjected to a one-way MLR in the presence or absence of varying concentrations of an L-arginine competitive inhibitor, NG-methyl-L-arginine (NMA: 50 to 500 [mu]M). When the MLR was performed in the presence of NMA (500 [mu]M), the production of IL-8 increased twofold (P P < 0.05), while MCP-1 and MIP-1[alpha] were not significantly altered. These findings suggest that NMA, an inhibitor of the L-arginine metabolic pathway, may regulate the production of specific C-X-C chemokines, IL-8 and ENA-78, during a MLR. In contrast, the production of MCP-1 and MIP-1[alpha], members of the C-C chemokine family, does not appear to be regulated by this inhibitor of the oxidative metabolism of L-arginine in the context of a MLR. | en_US |
dc.format.extent | 266976 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Regulation of Chemokine Production by the Oxidative Metabolism of L-Arginine in a Human Mixed Lymphocyte Reaction | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Medicine and Division of Pulmonary and Critical Care, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Medicine and Division of Pulmonary and Critical Care, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Medicine and Division of Pulmonary and Critical Care, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Medicine and Division of Pulmonary and Critical Care, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationother | The Theodor Kocher Institute, University of Bern, Bern, Switzerland | en_US |
dc.identifier.pmid | 8200045 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31513/1/0000435.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/cimm.1994.1155 | en_US |
dc.identifier.source | Cellular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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