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Effect of cell line on in vitro metal ion cytotoxicity

dc.contributor.authorWataha, John C.en_US
dc.contributor.authorHanks, Carl T.en_US
dc.contributor.authorSun, Zhi Linen_US
dc.date.accessioned2006-04-10T18:10:37Z
dc.date.available2006-04-10T18:10:37Z
dc.date.issued1994-05en_US
dc.identifier.citationWataha, John C., Hanks, Carl T., Sun, Zhilin (1994/05)."Effect of cell line on in vitro metal ion cytotoxicity." Dental Materials 10(3): 156-161. <http://hdl.handle.net/2027.42/31603>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6VP3-48G7047-BV/2/9a9f2aa514a2e26dd87ad152520888bben_US
dc.identifier.urihttps://hdl.handle.net/2027.42/31603
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7758857&dopt=citationen_US
dc.description.abstractObjectives. The choice of cell line for in vitro biological tests which assess the cytotoxicity of dental materials remains controversial, yet this issue is important because these tests are widely used to rate the biocompatibility of new and existing materials, and many different cell lines are commonly used. The Purpose of the current study was to quantify the responses of four cell lines (Balb/c 3T3, L929, ROS 17/2.8 and WI-38) to 14 metal ions which are released from dental materials, and relate these responses to the metabolic activity and population doubling times of these cells.Methods. Succinic dehydrogenase (SDH) activity was used to monitor metabolic activity and cytotoxic response.Results. The cell lines responded differently to most metal ions. In general, the Balb/c 3T3 line was the most sensitive, and the WI-38 line was the least sensitive. However, there were many exceptions depending on the metal ion. The passage number of the cells also affected the cytotoxic response. It was concluded that the cytotoxicity of materials which release metal ions will be significantly different depending on which cell line is selected and its passage number.Significance. Based on the findings that cell lines ranked the toxicities of the metal ions similarly, it seems reasonable to use these types of in vitro tests to rank the cytotoxicities of materials. However, if these types of tests are used to predict in vivo cytotoxicity, care should be taken to choose conditions and cells which are relevant.en_US
dc.format.extent673619 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleEffect of cell line on in vitro metal ion cytotoxicityen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumDepartment of Oral Medicine, Pathology and Surgery, University of Michigan School of Dentistry, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumDepartment of Oral Medicine, Pathology and Surgery, University of Michigan School of Dentistry, Ann Arbor, Michigan, USAen_US
dc.identifier.pmid7758857en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/31603/1/0000532.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0109-5641(94)90025-6en_US
dc.identifier.sourceDental Materialsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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