Positive and negative regulation of IL-2 gene expression: Role of multiple regulatory sites
dc.contributor.author | Zhang, Liqian | en_US |
dc.contributor.author | Nabel, Gary J. | en_US |
dc.date.accessioned | 2006-04-10T18:10:54Z | |
dc.date.available | 2006-04-10T18:10:54Z | |
dc.date.issued | 1994-05 | en_US |
dc.identifier.citation | Zhang, Liqian, Nabel, Gary J. (1994/05)."Positive and negative regulation of IL-2 gene expression: Role of multiple regulatory sites." Cytokine 6(3): 221-228. <http://hdl.handle.net/2027.42/31607> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WDF-4CF5G8Y-4K/2/b0c1b1b45150a28cdbacff6e058072d6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31607 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8054477&dopt=citation | en_US |
dc.description.abstract | Interleukin 2 (IL-2) is an important lymphokine required in the process of T cell activation, proliferation, clonal expansion and differentiation. The IL-2 gene displays both T cell specific and inducible expression: it is only expressed in CD4+ T cells after antigenic or mitogenic stimulation. Several cis-acting regulatory sites are required for induction of the IL-2 gene after stimulation. In this study, we have analysed the function of these cis-acting regulatory sites in the context of the native IL-2 enhancer and promoter sequence. The results of this study suggest that the NFAT (-276 to -261), the distal octamer (-256 to -248) and the proximal octamer (-75 to -66) sites not only act as enhancers of IL-2 gene transcription in the presence of cellular stimulation, but also have a silencing effect on IL-2 gene expression in resting cells. Two other sites display disparate effects on IL-2 gene expression in different T leukemia cell lines: the distal purine box (-291 to -277) and the proximal purine box sites (-145 to -128). Finally, the AP-1 (-186 to -176) and the [kappa]B sites (-206 to -195) respond to different cellular activation in EL4 cells. The AP-1 site mediated the response to PMA stimulation while the [kappa]B site responded to IL-1 stimulation. These data suggest that the regulation of IL-2 gene expression is a complex process and multiple cis-acting regulatory sites interact to exert different effects in T cells representative of alternative stages of differentiation. | en_US |
dc.format.extent | 825799 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Positive and negative regulation of IL-2 gene expression: Role of multiple regulatory sites | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Howard Hughes Medical Institute and Departments of Internal Medicine and Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI, USA. | en_US |
dc.contributor.affiliationum | Howard Hughes Medical Institute and Departments of Internal Medicine and Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI, USA. | en_US |
dc.identifier.pmid | 8054477 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31607/1/0000536.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/1043-4666(94)90016-7 | en_US |
dc.identifier.source | Cytokine | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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