Immunologic Detection of the Cellular Receptor for Urokinase Plasminogen Activator
dc.contributor.author | Mizukami, Ikuko F. | en_US |
dc.contributor.author | Garni-Wagner, Beth Ann | en_US |
dc.contributor.author | DeAngelo, L. Michael | en_US |
dc.contributor.author | Liebert, Monica | en_US |
dc.contributor.author | Flint, Andrew | en_US |
dc.contributor.author | Lawrence, David A. | en_US |
dc.contributor.author | Cohen, Robert L. | en_US |
dc.contributor.author | Todd, Robert F. III | en_US |
dc.date.accessioned | 2006-04-10T18:13:54Z | |
dc.date.available | 2006-04-10T18:13:54Z | |
dc.date.issued | 1994-04 | en_US |
dc.identifier.citation | Mizukami, Ikuko F., Garni-Wagner, Beth Ann, DeAngelo, L. Michael, Liebert, Monica, Flint, Andrew, Lawrence, David A., Cohen, Robert L., Todd, III, Robert F. (1994/04)."Immunologic Detection of the Cellular Receptor for Urokinase Plasminogen Activator." Clinical Immunology and Immunopathology 71(1): 96-104. <http://hdl.handle.net/2027.42/31653> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCK-45P0W50-16/2/6edad59d8e2e4b5c37c09b35cfdfac7d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31653 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8137563&dopt=citation | en_US |
dc.description.abstract | The cellular receptor for urokinase plasminogen activator (uPA-R) is a monomeric phosphatidylinositol-linked glycoprotein (gp40-65) that may contribute to the invasive capacity of tumor and inflammatory cells by focusing the activity of urokinase (uPA) in converting plasminogen to plasmin, a serine protease capable of degrading extracellular matrix proteins. The further characterization of uPA-R has been facilitated by our recent development of a monoclonal antibody, anti-Mo3f, specific for uPA-R. This mAb bound to uPA-R expressed by phorbol myristate acetate-stimulated U-937 cells and by NIH-3T3 cells permanently transfected with uPA-R cDNA. In competitive binding assays, anti-Mo3f inhibited the binding of fluorescein-conjugated uPA ligand to uPA-R expressed by U-937 cells and uPA-R transfectants; conversely, preexposure of cells to saturating quantities of exogenous uPA partially blocked the subsequent binding of anti-Mo3f mAb to uPA-R. Anti-Mo3f mAb was employed as the capture reagent in an ELISA for the quantitation of soluble forms of uPA-R (derived from U-937 cells and recombinant uPA-R) which had a sensitivity of approximately 4-12 ng/ml. Anti-Mo3f mAb was also applied as a serologic probe for the detection of uPA-R expressed by human tumor tissues. By immunoperoxidase staining, anti-Mo3f demonstrated positive tumor cell staining in 4 of 16 breast and 7 of 31 prostate carcinomas in formalin-fixed, paraffin-embedded specimens. These data indicate that the anti-M03f mAb detects an epitope proximate to or within the ligand binding domain (domain 1) of uPA-R and may be useful as a tool for the serologic detection of uPA-R in soluble form or associated with human tumors. | en_US |
dc.format.extent | 710223 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Immunologic Detection of the Cellular Receptor for Urokinase Plasminogen Activator | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.contributor.affiliationum | Simpson Memorial Institute, Departments of Internal Medicine, Surgery and Pathology and Divisions of Hematology, Oncology, Urology, University of Michigan Medical School, Ann Arbor, Michigan, USA; Cancer Research Institute, University of California at San Francisco, San Francisco, California, USA. | en_US |
dc.identifier.pmid | 8137563 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31653/1/0000587.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/clin.1994.1057 | en_US |
dc.identifier.source | Clinical Immunology and Immunopathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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