DT Diaphorase: Increased Enzyme Activity and mRNA Expression in Oxidant Stress of Skin
dc.contributor.author | Rees, Riley S. | en_US |
dc.contributor.author | Kingman, Gilson J. | en_US |
dc.contributor.author | Cashmer, Belinda | en_US |
dc.contributor.author | Gilmont, Robert R. | en_US |
dc.contributor.author | Reeves, Christopher | en_US |
dc.contributor.author | Welsh, Michael J. | en_US |
dc.contributor.author | Smith, Jr. , David J. | en_US |
dc.date.accessioned | 2006-04-10T18:15:12Z | |
dc.date.available | 2006-04-10T18:15:12Z | |
dc.date.issued | 1994-04 | en_US |
dc.identifier.citation | Rees, Riley S., Kingman, Gilson J., Cashmer, Belinda, Gilmont, Robert R., Reeves, Christopher, Welsh, Michael J., Smith, Jr., David J. (1994/04)."DT Diaphorase: Increased Enzyme Activity and mRNA Expression in Oxidant Stress of Skin." Journal of Surgical Research 56(4): 326-330. <http://hdl.handle.net/2027.42/31676> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-45P0G8F-3R/2/da40261afabf9b956c8191de99e07392 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31676 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8152225&dopt=citation | en_US |
dc.description.abstract | DT diaphorase is a flavoprotein that enzymaticly transfers two electrons from quinones as intermediate substrates and has been reported to increase its activity in the liver after exposure to toxicants. In this series of experiments, we tested the hypothesis that DT diaphorase also increases its activity after exposure to oxidants following gradient ischemia in skin. Using dorsal rat flaps, oxidant stress was induced immediately or during a 7-day period of preconditioning as a bipedicle flap before the distal attachment was divided. DT diaphorase activity ([Delta]Abs/min/100 g) or expression of message was measured during the period of preconditioning to determine the relationship between skin survival, enzyme activity, and expression of message. There was 4.7 +/- 0.8 cm of skin necrosis in the distal end of acute flaps while the preconditioned flaps had no skin necrosis after the distal attachment was divided. In the acute flaps, the DT diaphorase activity was equal throughout the flap for the first 6 hr. After 24 hr of ischemia, the DT diaphorase activity was significantly higher in the proximal end of the flap (1.83 +/- 0.21 [Delta]Abs/min/100 g) than that in the distal end (0.005 +/- 0.01 [Delta]Abs/min/100 g), which was significant (P P < 0.05). Maximal enzyme induction of DT diaphorase activity occurred after 4 days of preconditioning and correlated with the maximal expression of mRNA. These studies provide the first evidence that DT diaphorase enzyme activity is inducible after oxidant stress. The data also suggests that DT activity remains elevated for at least 6 hr of ischemia and may be a potential source of anti-oxidant activity in ischemic skin. | en_US |
dc.format.extent | 320921 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | DT Diaphorase: Increased Enzyme Activity and mRNA Expression in Oxidant Stress of Skin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.contributor.affiliationum | Department of Surgery, Section of Plastic and Reconstructive Surgery, and Department of Anatomy & Cell Biology, University of Michigan, 1500 East Medical Center Drive, Room 2130, Taubman Center, Ann Arbor, Michigan 48109-0340; and McAuley Health Sciences Center, Ann Arbor, Michigan 48103 | en_US |
dc.identifier.pmid | 8152225 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31676/1/0000612.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/jsre.1994.1050 | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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