Nucleotide Sequence Analysis of 77.7 kb of the Human V[beta] T-Cell Receptor Gene Locus: Direct Primer-Walking Using Cosmid Template DNAs
dc.contributor.author | Slightom, Jerry L. | en_US |
dc.contributor.author | Siemieniak, David R. | en_US |
dc.contributor.author | Sieu, Leang C. | en_US |
dc.contributor.author | Koop, Ben F. | en_US |
dc.contributor.author | Hood, Leroy | en_US |
dc.date.accessioned | 2006-04-10T18:16:36Z | |
dc.date.available | 2006-04-10T18:16:36Z | |
dc.date.issued | 1994-03-15 | en_US |
dc.identifier.citation | Slightom, Jerry L., Siemieniak, David R., Sieu, Leang C., Koop, Ben F., Hood, Leroy (1994/03/15)."Nucleotide Sequence Analysis of 77.7 kb of the Human V[beta] T-Cell Receptor Gene Locus: Direct Primer-Walking Using Cosmid Template DNAs." Genomics 20(2): 149-168. <http://hdl.handle.net/2027.42/31701> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-45NJY06-50/2/c8cab5d4442de1cf18cc0946959627ed | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31701 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8020962&dopt=citation | en_US |
dc.description.abstract | The nucleotide sequence of 77.7 kb from the human T-cell receptor [beta]-chain locus was determined directly from three overlapping cosmid clones using the primer-walking approach. Computer-aided analyses of this sequence reveal the presence of at least 11 genic regions that are closely related to the human T-cell receptor [beta] variable region (TCRBV) gene family. These include five germline sequences that have previously been determined, V[beta]21.2, V[beta]8.1, V[beta]8.2, V[beta]8.3, and V[beta]16, and four whose sequences have partially been determined at the mRNA level, V[beta]6, V[beta]23, V[beta]12.2, V[beta]24. The two remaining V[beta] Tcr-related sequences have eluded discovery by cDNA and RT-PCR cloning and genomic blot hybridization methods. These two V[beta] Tcr-related genes lack >75% nucleotide sequence identity with any other V[beta] Tcr gene member and therefore, by convention, are referred to as new subfamily members V[beta]25 and V[beta]26. This lack of shared identity with other subfamily members explains why they were not detected by hybridization. The promoter regions of these V[beta] Tcr genes contain the conserved Tcr decamer element located between 80 and 110 bp 5' of the translation start site, generally near a putative TATAA promoter element. Our sequence analysis also reveals that a 3.3-kb duplication unit was involved in the recombination event that produced the closely related V[beta]8.1 and 8.2 gene subfamily members. This sequenced region of the V[beta] locus contains an average number of repetitive DNA elements (21 Alu, three L1, three MER, and three retrovirus-related elements). | en_US |
dc.format.extent | 1323913 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Nucleotide Sequence Analysis of 77.7 kb of the Human V[beta] T-Cell Receptor Gene Locus: Direct Primer-Walking Using Cosmid Template DNAs | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Molecular Biology Unit 7242, The Upjohn Company, Kalamazoo, Michigan 49007; Human Genome Center, The University of Michigan, Ann Arbor, Michigan 48109-0650; Department of Biology, Center for Environmental Health, University of Victoria, Box 1700, Victoria, British Columbia V8W 2Y2, Canada; and Department of Molecular Biotechnology, University of Washington, 4909 25th Avenue N.E., Seattle, Washington 98105 | en_US |
dc.contributor.affiliationum | Molecular Biology Unit 7242, The Upjohn Company, Kalamazoo, Michigan 49007; Human Genome Center, The University of Michigan, Ann Arbor, Michigan 48109-0650; Department of Biology, Center for Environmental Health, University of Victoria, Box 1700, Victoria, British Columbia V8W 2Y2, Canada; and Department of Molecular Biotechnology, University of Washington, 4909 25th Avenue N.E., Seattle, Washington 98105 | en_US |
dc.contributor.affiliationum | Molecular Biology Unit 7242, The Upjohn Company, Kalamazoo, Michigan 49007; Human Genome Center, The University of Michigan, Ann Arbor, Michigan 48109-0650; Department of Biology, Center for Environmental Health, University of Victoria, Box 1700, Victoria, British Columbia V8W 2Y2, Canada; and Department of Molecular Biotechnology, University of Washington, 4909 25th Avenue N.E., Seattle, Washington 98105 | en_US |
dc.contributor.affiliationum | Molecular Biology Unit 7242, The Upjohn Company, Kalamazoo, Michigan 49007; Human Genome Center, The University of Michigan, Ann Arbor, Michigan 48109-0650; Department of Biology, Center for Environmental Health, University of Victoria, Box 1700, Victoria, British Columbia V8W 2Y2, Canada; and Department of Molecular Biotechnology, University of Washington, 4909 25th Avenue N.E., Seattle, Washington 98105 | en_US |
dc.contributor.affiliationum | Molecular Biology Unit 7242, The Upjohn Company, Kalamazoo, Michigan 49007; Human Genome Center, The University of Michigan, Ann Arbor, Michigan 48109-0650; Department of Biology, Center for Environmental Health, University of Victoria, Box 1700, Victoria, British Columbia V8W 2Y2, Canada; and Department of Molecular Biotechnology, University of Washington, 4909 25th Avenue N.E., Seattle, Washington 98105 | en_US |
dc.identifier.pmid | 8020962 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31701/1/0000637.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/geno.1994.1149 | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.