Hypoxic-Ischemic Brain Injury Stimulates Glial Fibrillary Acidic Protein mRNA and Protein Expression in Neonatal Rats
dc.contributor.author | Burtrum, Douglas | en_US |
dc.contributor.author | Silverstein, Faye Sarah | en_US |
dc.date.accessioned | 2006-04-10T18:18:30Z | |
dc.date.available | 2006-04-10T18:18:30Z | |
dc.date.issued | 1994-03 | en_US |
dc.identifier.citation | Burtrum, Douglas, Silverstein, Faye S. (1994/03)."Hypoxic-Ischemic Brain Injury Stimulates Glial Fibrillary Acidic Protein mRNA and Protein Expression in Neonatal Rats." Experimental Neurology 126(1): 112-118. <http://hdl.handle.net/2027.42/31734> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFG-45NJTR0-4R/2/de2eaa1ea091bc06d1dcf0e06c6646f9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31734 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8157121&dopt=citation | en_US |
dc.description.abstract | Accumulation of glial fibrillary acidic protein xk(G-FAP) in reactive astrocytes is a characteristic neuropathologic feature of ischemic brain injury. We examined injury-induced changes in GFAP mRNA and protein in a well-characterized model of focal hypoxicischemic injury in perinatal rodent brain. Postnatal Day (PND) 7 rats underwent right carotid artery ligation followed by 2.5 h exposure to 8% oxygen, which results in injury to ipsilateral cortex, hippocampus, and striatum in the majority of animals. Using Northern analysis, we assayed GFAP mRNA in samples from the lesioned and contralateral hemispheres of animals killed 1 h to 14 days later, and from animals treated with the neuroprotective glutamate antagonist MK-801. GFAP immunoreactivity in tissue homagenates from the lesioned and contralateral hemispheres was also compared with an immunoblot assay. One and 4 h posthypoxia GFAP mRNA expression was barely detectable. In the lesioned cortex, increased GFAP mRNA was detected at 24 h postinjury; over the next 2 weeks GFAP mRNA was consistently higher (at least 2-fold) in lesioned than in contralateral cortex. In contrast, in lesioned hippocampus and striatum, consistent increases in GFAP mHNA were first detected on PND 12. Immunoassays of GFAP demonstrated early (PND 8) and sustained (to PND 21) up to 10-fold increases in lesioned cortex, hippocampus, and striatum. In this perinatal stroke model regionally specific increases in GFAP mRNA expression and GFAP immunoreactivity are detected in the first 2 weeks after hypoxicischemic injury; intrinsic properties of glia and/or neurons in different brain regions may influence the timing and mag- xe03nitude of stimulation of this response. | en_US |
dc.format.extent | 591219 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Hypoxic-Ischemic Brain Injury Stimulates Glial Fibrillary Acidic Protein mRNA and Protein Expression in Neonatal Rats | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Psychiatry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Pediatrics and Neurology, University of Michigan, Ann Arbor, Michigan 48109-0570 | en_US |
dc.contributor.affiliationum | Departments of Pediatrics and Neurology, University of Michigan, Ann Arbor, Michigan 48109-0570 | en_US |
dc.identifier.pmid | 8157121 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31734/1/0000673.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/exnr.1994.1047 | en_US |
dc.identifier.source | Experimental Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.