Modulation of opioid signal transduction in SH-SY5Y neural cells by differentiating agents: Concurrent mu receptor upregulation and effector desensitization by phorbol ester
dc.contributor.author | Lin H. , | en_US |
dc.contributor.author | Carter B. D. , | en_US |
dc.contributor.author | Haas K. F. , | en_US |
dc.contributor.author | Medzihradsky F. , | en_US |
dc.date.accessioned | 2006-04-10T18:20:18Z | |
dc.date.available | 2006-04-10T18:20:18Z | |
dc.date.issued | 1994-02-21 | en_US |
dc.identifier.citation | Lin H., , Carter B. D., , Haas K. F., , Medzihradsky F., (1994/02/21)."Modulation of opioid signal transduction in SH-SY5Y neural cells by differentiating agents: Concurrent mu receptor upregulation and effector desensitization by phorbol ester." Regulatory Peptides 53(Supplement 1): S21-S22. <http://hdl.handle.net/2027.42/31768> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0S-47W0D4P-F/2/8199a897af145c8fe38caea35dd8fe3f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31768 | |
dc.description.abstract | Long-term exposure of SH-SY5Y human neural cells to retinoic acid (RA) increased the binding of [3H]DAMGO and [3H]DPDPE by up to 3-fold compared to membranes of untreated cells. In contrast, incubation of the cells with phorbol ester (TPA) selectively up-regulated the binding of [3H]DAMGO. RA enhanced the maximal inhibition of cAMP formation in SH-SY5Y cells by both DAMGO and DPDPE from 20% for both opioids in control cells to 75% and 50%, respectively. On the other hand, the effect of TPA was limited to a marginal increase in the inhibition of cAMP formation by DAMGO. Parameters of GTP[gamma]35S binding as well as low-Km GTPase activity revealed no deterioration in the structure or function of total G protein in the TPA-treated cells, and initial Western blots showed no difference in the cell content of Go. Ongoing experiments are focusing on the covalent modification of specific G protein subtypes involved in [mu] and [delta] opioid signal transduction in SH-SY5Y cells differentiated by TPA. | en_US |
dc.format.extent | 183253 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Modulation of opioid signal transduction in SH-SY5Y neural cells by differentiating agents: Concurrent mu receptor upregulation and effector desensitization by phorbol ester | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA. | en_US |
dc.contributor.affiliationum | Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA. | en_US |
dc.contributor.affiliationum | Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA. | en_US |
dc.contributor.affiliationum | Dept of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Dept of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31768/1/0000709.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-0115(94)90221-6 | en_US |
dc.identifier.source | Regulatory Peptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.