Bacteriophage Lambda N-Dependent Transcription Antitermination : Competition for an RNA Site May Regulate Antitermination
dc.contributor.author | Patterson, Thomas A. | en_US |
dc.contributor.author | Zhang, Zhaoshan | en_US |
dc.contributor.author | Baker, Teresa | en_US |
dc.contributor.author | Johnson, Linda L. | en_US |
dc.contributor.author | Friedman, David I. | en_US |
dc.contributor.author | Court, Donald L. | en_US |
dc.date.accessioned | 2006-04-10T18:20:52Z | |
dc.date.available | 2006-04-10T18:20:52Z | |
dc.date.issued | 1994-02-10 | en_US |
dc.identifier.citation | Patterson, Thomas A., Zhang, Zhaoshan, Baker, Teresa, Johnson, Linda L., Friedman, David I., Court, Donald L. (1994/02/10)."Bacteriophage Lambda N-Dependent Transcription Antitermination : Competition for an RNA Site May Regulate Antitermination." Journal of Molecular Biology 236(1): 217-228. <http://hdl.handle.net/2027.42/31780> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WK7-45NSKJ9-M1/2/aa6432195a964a10ec0a96ecad195b7a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31780 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8107107&dopt=citation | en_US |
dc.description.abstract | Bacteriophage [lambda] controls the expression of its early genes in a temporal manner by a series of transcription termination and antitermination events. This antitermination requires the [lambda] N protein as well as host proteins called Nus, and cis-acting sites called nut. Following transcription of the nut site, N and Nus proteins bind to the nut RNA and modify the transcription complex to a termination-resistant form. The nut site is a composite of at least two components; one is the boxB hairpin structure which interacts with N. The other is boxA, a nine-nucleotide sequence upstream of boxB. To understand more about the formation of the antitermination complex, we have characterized the effect of point mutations in and deletions of boxA on antitermination. Point mutations in boxA were found to either enhance or reduce N-mediated antitermination. Several boxA deletions, on the other hand, had little effect on antitermination other than to eliminate the requirement for the NusB host protein. To explain these observations, we propose that at least two factors complete to interact with boxA, NusB and an inhibitor of the antitermination reaction. In addition, we propose that NusB is required to prevent the inhibitor from binding at boxA. The results with various nusB and boxA mutations can be explained by this model of competition between NusB and an inhibitor for boxA RNA. | en_US |
dc.format.extent | 759346 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Bacteriophage Lambda N-Dependent Transcription Antitermination : Competition for an RNA Site May Regulate Antitermination | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Department of Microbiology and Immunology Ann Arbor, MI 48109-0620, U.S.A. | en_US |
dc.contributor.affiliationum | University of Michigan Department of Microbiology and Immunology Ann Arbor, MI 48109-0620, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Chromosome Biology, ABL-Basic Research Program NCI-Frederick Cancer Research and Development Center P.O. Box B, Frederick, MD 21702, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Chromosome Biology, ABL-Basic Research Program NCI-Frederick Cancer Research and Development Center P.O. Box B, Frederick, MD 21702, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Chromosome Biology, ABL-Basic Research Program NCI-Frederick Cancer Research and Development Center P.O. Box B, Frederick, MD 21702, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Chromosome Biology, ABL-Basic Research Program NCI-Frederick Cancer Research and Development Center P.O. Box B, Frederick, MD 21702, U.S.A. | en_US |
dc.identifier.pmid | 8107107 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31780/1/0000721.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/jmbi.1994.1131 | en_US |
dc.identifier.source | Journal of Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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