H2O2-Induced Increases in Cellular F-Actin Occur without Increases in Actin Nucleation Activity
dc.contributor.author | Omann G. M. , | en_US |
dc.contributor.author | Harter J. M. , | en_US |
dc.contributor.author | Burger J. M. , | en_US |
dc.contributor.author | Hinshaw D. B. , | en_US |
dc.date.accessioned | 2006-04-10T18:23:00Z | |
dc.date.available | 2006-04-10T18:23:00Z | |
dc.date.issued | 1994-02 | en_US |
dc.identifier.citation | Omann G. M., , Harter J. M., , Burger J. M., , Hinshaw D. B., (1994/02)."H2O2-Induced Increases in Cellular F-Actin Occur without Increases in Actin Nucleation Activity." Archives of Biochemistry and Biophysics 308(2): 407-412. <http://hdl.handle.net/2027.42/31818> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-45P0HM3-CP/2/1bb8cdc26a27dfb60df2e73354af6d77 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31818 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8109969&dopt=citation | en_US |
dc.description.abstract | Previous work has shown that H2O2 causes an increase in polymerized actin (F-actin) inside cells. To test the hypothesis that increased polymerization resulted from a mechanism involving increased actin nucleation activity, we employed methods utilizing pyrene-labeled actin to quantify the actin nucleation activity of cell lysates and N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD)-phallacidin binding assays to quantify the amount of F-actin in P388D1 cells. H2O2 increased polymerized actin (NBD-phallacidin assay) in a dose-dependent manner with an effective dose giving 50% response (ED50) [asymp] 1 mM. Five millimolar H2O2 caused a 1.6-fold increase in NBD-phallacidin staining. In contrast, actin nucleation activity decreased in a dose-dependent manner with a similar ED50. Five millimolar H2O2 caused a 30-40% decrease in actin nucleation activity. The effect was rapid, occurring within 5 min of H2O2 addition. The results indicate that H2O2 causes cytoskeletal changes that enhance NBD-phallacidin binding without increasing actin nucleation activity. Fractionation studies showed that the nucleation activity in H2O2-treated cells and controls sedimented with the Triton X-100-insoluble cytoskeleton, and the cytosolic fraction appeared to contain an inhibitor of actin polymerization. | en_US |
dc.format.extent | 486467 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | H2O2-Induced Increases in Cellular F-Actin Occur without Increases in Actin Nucleation Activity | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Surgery and Biological Chemistry, University of Michigan Medical School and VA Medical Center, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, MI, USA. | en_US |
dc.contributor.affiliationum | VA Medical Center, Ann Arbor, Michigan, USA; Department of Surgery, University of Michigan Medical School, Ann Arbor, MI, USA. | en_US |
dc.contributor.affiliationother | VA Medical Center, Ann Arbor, Michigan, USA. | en_US |
dc.identifier.pmid | 8109969 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31818/1/0000764.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/abbi.1994.1057 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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