Callitachykinin I and II, two novel myotropic peptides isolated from the blowfly, Calliphora vomitoria, that have resemblances to tachykinins
dc.contributor.author | Tomas Lundquist, C. | en_US |
dc.contributor.author | Clottens, Frank L. | en_US |
dc.contributor.author | Mark Holman, G. | en_US |
dc.contributor.author | Nichols, Ruthann | en_US |
dc.contributor.author | Nachman, Ronald J. | en_US |
dc.contributor.author | Nassel, Dick R. | en_US |
dc.date.accessioned | 2006-04-10T18:29:43Z | |
dc.date.available | 2006-04-10T18:29:43Z | |
dc.date.issued | 1994 | en_US |
dc.identifier.citation | Tomas Lundquist, C., Clottens, Frank L., Mark Holman, G., Nichols, Ruthann, Nachman, Ronald J., Nassel, Dick R. (1994)."Callitachykinin I and II, two novel myotropic peptides isolated from the blowfly, Calliphora vomitoria, that have resemblances to tachykinins." Peptides 15(5): 761-768. <http://hdl.handle.net/2027.42/31940> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0M-47T93WC-26/2/55f26110bbaf74e6411cfcb7f6ab3b10 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31940 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7984492&dopt=citation | en_US |
dc.description.abstract | Two peptides, related to the locust myotropic peptides locustatachykinin I-IV, were isolated from the blowfly Calliphora vomitoria. Whole, frozen flies were used for extraction with acidified methanol. A cockroach hindgut muscle contraction bioassay was used for monitoring fractions during subsequent purification steps. A series of eight different high performance liquid chromatography column systems was required to obtain optically pure peptides. Two peptides were isolated and their sequences determined by Edman degradation and confirmed by mass spectrometry and chemical synthesis as APTAFYGVR-NH2 and GLGNNAFVGVR-NH2. They were named callitachykinin I and II. The peptides have sequence similarities to the locustatachykinins and vertebrate tachykinins. Both callitachykinins were recognized by an antiserum to locustatachykinin I in enzyme-linked immunosorbent assay (ELISA) tests and callitachykinin II was additionally recognized by an antiserum to the vertebrate tachykinin kassinin, suggesting that immunolabeling of blowfly neurons with these antisera is due to neuronal callitachykinins. | en_US |
dc.format.extent | 786435 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Callitachykinin I and II, two novel myotropic peptides isolated from the blowfly, Calliphora vomitoria, that have resemblances to tachykinins | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA | en_US |
dc.contributor.affiliationother | Department of Zoology, Stockholm University, S-10691, Stockholm, Sweden | en_US |
dc.contributor.affiliationother | Food Animal Protection Research Laboratory, United States Department of Agriculture, College Station, TX 77845, USA | en_US |
dc.contributor.affiliationother | Food Animal Protection Research Laboratory, United States Department of Agriculture, College Station, TX 77845, USA | en_US |
dc.contributor.affiliationother | Food Animal Protection Research Laboratory, United States Department of Agriculture, College Station, TX 77845, USA | en_US |
dc.contributor.affiliationother | Department of Zoology, Stockholm University, S-10691, Stockholm, Sweden | en_US |
dc.identifier.pmid | 7984492 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31940/1/0000893.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0196-9781(94)90027-2 | en_US |
dc.identifier.source | Peptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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