Coupling an [alpha]2-Adrenergic receptor peptide to G-protein: A new photolabeling agent
dc.contributor.author | Taylor, J. M. | en_US |
dc.contributor.author | Jacob-Mosier, G. G. | en_US |
dc.contributor.author | Lawton, Richard G. | en_US |
dc.contributor.author | Neubig, Richard R. | en_US |
dc.date.accessioned | 2006-04-10T18:29:46Z | |
dc.date.available | 2006-04-10T18:29:46Z | |
dc.date.issued | 1994 | en_US |
dc.identifier.citation | Taylor, J. M., Jacob-Mosier, G. G., Lawton, R. G., Neubig, R. R. (1994)."Coupling an [alpha]2-Adrenergic receptor peptide to G-protein: A new photolabeling agent." Peptides 15(5): 829-834. <http://hdl.handle.net/2027.42/31941> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0M-47T93WC-2K/2/e70b02954e8340faf72cfef3c38e48ae | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31941 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7984502&dopt=citation | en_US |
dc.description.abstract | A photoreactive derivative of a tetradecapeptide G-protein activator (peptide Q) derived from the [alpha]2-adrenergic receptor was designed and used to label purified G-protein (Go/Gi). N-bromoacetyl-N'-(3-diazopyruvoyl)-m-phenylene-diamine (Br-DAP) was conjugated to the C-terminal cysteine of peptide Q. The DAP-modified peptide Q (DAP-Q) specifically incorporated into the a subunit of Go. The incorporation of DAP-Q into [alpha]o was blocked by unmodified Q peptide (IC50 = 15 +/- 6 [mu]M; n = 4). Photolysis of sixfold higher concentrations of DAP-Q with ovalbumin or bovine albumin failed to produce cross-linked products. Br-DAP should prove useful in detecting mutual contact sites between peptides and their binding proteins. | en_US |
dc.format.extent | 558738 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Coupling an [alpha]2-Adrenergic receptor peptide to G-protein: A new photolabeling agent | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Internal Medicine (Hypertension), University of Michigan, Ann Arbor, MI 48109, USA. | en_US |
dc.identifier.pmid | 7984502 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31941/1/0000894.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0196-9781(94)90038-8 | en_US |
dc.identifier.source | Peptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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